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CXCR1 elisa kit :: Mouse CXC-chemokine receptor 1 ELISA Kit

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Catalog # MBS728400
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CXC-chemokine receptor 1 (CXCR1), ELISA Kit

Popular Item
Full Product Name

Mouse CXC-chemokine receptor 1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
146929
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CXCR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CXCR1purchase
MBS728400 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CXC-chemokine receptor 1 (CXCR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCR1. The ELISA analytical biochemical technique of the MBS728400 kit is based on CXCR1 antibody-CXCR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CXCR1 elisa kit
Intended Uses: This CXCR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CXCR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CXCR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCR1 antibody and an CXCR1-HRP conjugate. The assay sample and buffer are incubated together with CXCR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR1 concentration since CXCR1 from samples and CXCR1-HRP conjugate compete for the anti-CXCR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR1 from the sample, fewer sites are left to bind CXCR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CXCR1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of CXCR1 elisa kit
CXCR1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS728400. Click to request current manual
NCBI/Uniprot data below describe general gene information for CXCR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,791 Da
NCBI Official Full Name
CXCR1
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 1
NCBI Official Symbol
CXCR1  [Similar Products]
NCBI Official Synonym Symbols
C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1
  [Similar Products]
NCBI Protein Information
C-X-C chemokine receptor type 1; CXC-R1; CXCR-1; IL-8R A; IL-8 receptor type 1; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A; high affinity interleukin-8 receptor A
UniProt Protein Name
C-X-C chemokine receptor type 1
UniProt Synonym Protein Names
CDw128a; High affinity interleukin-8 receptor A; IL-8R A; IL-8 receptor type 1; CD_antigen: CD181
Protein Family
UniProt Gene Name
CXCR1  [Similar Products]
UniProt Synonym Gene Names
CMKAR1; IL8RA; CXC-R1; CXCR-1; IL-8R A  [Similar Products]
UniProt Entry Name
CXCR1_HUMAN
NCBI Summary for CXCR1
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008]
UniProt Comments for CXCR1
IL8RA: Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Receptor, cytokine

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; interleukin-8 receptor activity; chemokine receptor activity; interleukin-8 binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; receptor internalization; dendritic cell chemotaxis; chemotaxis; inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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