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CYFRA elisa kit :: Human CYFRA ELISA Kit

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Catalog # MBS494749-211-10
Unit / Price DISCONTINUED
Typical Testing Data/Standard Curve (for reference only)
Product Name

CYFRA, ELISA Kit

Also Known As

CYFRA 21-1 ELISA

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Samples
Human serum
Intended Uses
The CYFRA 21-1 EIA kit is intended for the determination of soluble cytokeratin 19 fragments in human serum. The assay is to be used as an aid in monitoring disease progression during the course of disease and treatment in lung cancer patients. Serial testing for patient CYFRA 21-1 assay values should be used in conjunction with other clinical methods used for monitoring lung cancer.
Preparation and Storage
2 - 8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYFRA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYFRApurchase
MBS494749-211-10 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CYFRA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYFRA. The ELISA analytical biochemical technique of the MBS494749-211-10 kit is based on CYFRA antibody-CYFRA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYFRA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYFRA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYFRA elisa kit
Principle of the assay: The CYFRA 21-1 EIA is a solid phase, non-competitive immunoassay based on two monoclonal antibodies (derived from mice) directed against two separate antigenic determinants of soluble fragments of cytokeratin 19 (7-8). Calibrators, controls and patient samples are incubated together with biotinylated Anti-CYFRA 21-1 MAb and horseradish peroxidase (HRP) labeled Anti-CYFRA 21-1 MAb in streptavidin coated microstrips. After washing, buffered Substrate/Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetramethylbenzidine) is added to each well and the enzyme reaction is allowed to proceed. During the enzyme reaction a blue color will develop if antigen is present. The intensity of the color development is proportional to the amount of CYFRA 21-1 present in the samples. The color intensity is determined in a microplate spectrophotometer at 620 nm (or optionally at 405 nm after addition of Stop Solution). Calibration curves are constructed for each assay by plotting absorbance value versus the concentration for each calibrator. The CYFRA 21-1 concentrations of patient samples are then read from the calibration curve.
Product Categories/Family for CYFRA elisa kit

Typical Testing Data/Standard Curve (for reference only) of CYFRA elisa kit
CYFRA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS494749-211-10. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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