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CYP11A1 elisa kit :: Rat Cytochrome P450 11A1 (CYP11A1) ELISA Kit

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Catalog # MBS452451
Unit / Price
  48-Strip-Wells  /  $425 +1 FREE 8GB USB
  96-Strip-Wells  /  $570 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,040 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cytochrome P450 11A1 (CYP11A1), ELISA Kit

Full Product Name

Rat Cytochrome P450 11A1 (CYP11A1) ELISA Kit

Product Synonym Names
CYP11A; P450SCC; Cholesterol desmolase; Cholesterol Side Chain Cleavage Enzyme; Cytochrome P450 Family 11 Subfamily A Polypeptide 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P14137
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CYP11A1. No significant cross-reactivity or interference between CYP11A1 and analogues was observed.
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.064ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP11A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP11A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP11A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYP11A1purchase
MBS452451 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 11A1 (CYP11A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP11A1. The ELISA analytical biochemical technique of the MBS452451 kit is based on CYP11A1 antibody-CYP11A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP11A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP11A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP11A1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYP11A1 in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP11A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYP11A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP11A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYP11A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CYP11A1 elisa kit
CYP11A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS452451. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP11A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60,586 Da
NCBI Official Full Name
cholesterol side-chain cleavage enzyme, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp11a1  [Similar Products]
NCBI Official Synonym Symbols
Cyp11a; Cypxia1; P450scc; P450(scc)
  [Similar Products]
NCBI Protein Information
cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Protein Name
Cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Synonym Protein Names
CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)
UniProt Gene Name
Cyp11a1  [Similar Products]
UniProt Synonym Gene Names
Cyp11a; Cyp11a-1  [Similar Products]
NCBI Summary for CYP11A1
monooxygenase that catalyzes synthesis of cholesterol and steroids [RGD, Feb 2006]
UniProt Comments for CYP11A1
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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