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CYP11B1 elisa kit :: Rat Cytochrome P450 11B1 (CYP11B1) ELISA Kit

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Catalog # MBS453205
Unit / Price
  48-Strip-Wells  /  $425 +1 FREE 8GB USB
  96-Strip-Wells  /  $570 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,040 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cytochrome P450 11B1 (CYP11B1), ELISA Kit

Full Product Name

Rat Cytochrome P450 11B1 (CYP11B1) ELISA Kit

Product Synonym Names
CPN1; CYP11B; FHI; CYPXIB1; S11BH; 11-Beta-Hydroxylase; Cytochrome P450 Family 11 Subfamily B Polypeptide 1; Steroid 11-beta-hydroxylase; Cytochrome P-450c11
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P15393
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of CYP11B1. No significant cross-reactivity or interference between CYP11B1 and analogues was observed.
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
0.29ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP11B1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP11B1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP11B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYP11B1purchase
MBS453205 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 11B1 (CYP11B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP11B1. The ELISA analytical biochemical technique of the MBS453205 kit is based on CYP11B1 antibody-CYP11B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP11B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP11B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP11B1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYP11B1 in rat tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP11B1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYP11B1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP11B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYP11B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CYP11B1 elisa kit
CYP11B1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS453205. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP11B1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,459 Da
NCBI Official Full Name
Cytochrome P450 11B1, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily b, polypeptide 1
NCBI Official Symbol
Cyp11b1  [Similar Products]
NCBI Official Synonym Symbols
Cp11ba; RATCP11BA
  [Similar Products]
NCBI Protein Information
cytochrome P450 11B1, mitochondrial
UniProt Protein Name
Cytochrome P450 11B1, mitochondrial
UniProt Synonym Protein Names
CYPXIB1; Cytochrome P450(11 beta)-DS; Cytochrome P450C11; Steroid 11-beta-hydroxylase (EC:1.14.15.4)
Protein Family
UniProt Gene Name
Cyp11b1  [Similar Products]
UniProt Synonym Gene Names
Cyp11b-1  [Similar Products]
NCBI Summary for CYP11B1
catalyzes the conversion of 11-deoxycorticosterone to corticosterone [RGD, Feb 2006]
UniProt Comments for CYP11B1
Forms corticosterone from 11-deoxycorticosterone.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with CYP11B1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >55 publications with CYP11B1 and Mixed
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