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CYP11B2 elisa kit :: Human cytochrome P450, family 11, subfamily B, polypeptide 2 ELISA Kit

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Catalog # MBS933334
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Typical Testing Data/Standard Curve (for reference only)
Product Name

cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), ELISA Kit

Full Product Name

Human Cytochrome P450 11B2, mitochondrial, CYP11B2 ELISA Kit

Product Synonym Names
Human Cytochrome P450 11B2; mitochondrial (CYP11B2) ELISA kit; ALDOS; CPN2; CYP11B; CYP11BL; P-450C18; P450C18; P450aldo; aldosterone synthase; cytochrome P450; subfamily XIB (steroid 11-beta-hydroxylase); polypeptide 2; mitochondrial cytochrome P450; family 11; ; cytochrome P450; family 11; subfamily B; polypeptide 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS933334 Testing Data
OMIM
610600
3D Structure
ModBase 3D Structure for P19099
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CYP11B2. No significant cross-reactivity or interference between Human CYP11B2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
47 pg/ml-3000 pg/ml
Sensitivity
11.7 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Lipogenesis and lipometabolism
Protein Biological Process 3
Lipid metabolism
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP11B2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CYP11B2purchase
MBS933334 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP11B2. The ELISA analytical biochemical technique of the MBS933334 kit is based on CYP11B2 antibody-CYP11B2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP11B2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP11B2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP11B2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP11B2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP11B2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP11B2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP11B2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CYP11B2 elisa kit
CYP11B2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS933334. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP11B2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,560 Da
NCBI Official Full Name
cytochrome P450 11B2, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily B, polypeptide 2
NCBI Official Symbol
CYP11B2  [Similar Products]
NCBI Official Synonym Symbols
CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo
  [Similar Products]
NCBI Protein Information
cytochrome P450 11B2, mitochondrial; cytochrome P-450C18; aldosterone synthase; cytochrome P-450Aldo; steroid 11-beta-monooxygenase; steroid 11-beta/18-hydroxylase; aldosterone-synthesizing enzyme; steroid 18-hydroxylase, aldosterone synthase, P450C18, P450aldo; mitochondrial cytochrome P450, family 11, subfamily B, polypeptide 2; cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2
UniProt Protein Name
Cytochrome P450 11B2, mitochondrial
UniProt Synonym Protein Names
Aldosterone synthase (EC:1.14.15.4, EC:1.14.15.5); ALDOS; Aldosterone-synthesizing enzyme; CYPXIB2; Cytochrome P-450Aldo; Cytochrome P-450C18; Steroid 18-hydroxylase
Protein Family
UniProt Gene Name
CYP11B2  [Similar Products]
UniProt Synonym Gene Names
ALDOS  [Similar Products]
UniProt Entry Name
C11B2_HUMAN
NCBI Summary for CYP11B2
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP11B2
CYP11B2: Preferentially catalyzes the conversion of 11- deoxycorticosterone to aldosterone via corticosterone and 18- hydroxycorticosterone. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency); also known as aldosterone deficiency due to defect in 18- hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18- hydroxycorticosterone, is low or normal. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency). CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18- hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum. Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1). It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.15.5; EC 1.14.15.4; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Mitochondrial

Chromosomal Location of Human Ortholog: 8q21-q22

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: corticosterone 18-monooxygenase activity; steroid 11-beta-monooxygenase activity; iron ion binding; heme binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; mineralocorticoid biosynthetic process; renal water homeostasis; C21-steroid hormone biosynthetic process; sodium ion homeostasis; aldosterone mediated regulation of blood volume; sterol metabolic process; aldosterone biosynthetic process; potassium ion homeostasis; cellular response to hormone stimulus

Disease: Corticosterone Methyloxidase Type Ii Deficiency; Corticosterone Methyloxidase Type I Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with CYP11B2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Adrenal Gland Antibodies  >378 publications with CYP11B2 and Adrenal Gland
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