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CYP17A1 elisa kit :: Human cytochrome P450, family 17, subfamily A, polypeptide 1 ELISA Kit

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Catalog # MBS702060
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1), ELISA Kit

Popular Item
Also Known As

Human 17-alpha-hydroxylase, 17-alpha-OH ELISA Kit

Product Synonym Names
Human 17-alpha-hydroxylase; 17-alpha-OH ELISA Kit; RP11-753C18.1; CPT7; CYP17; P450C17; S17AH; cytochrome P450; subfamily XVII (steroid 17-alpha-hydroxylase); adrenal hyperplasia; cytochrome p450 XVIIA1; steroid 17-alpha-hydroxylase/17; 20 lyase; steroi; cytochrome P450; family 17; subfamily A; polypeptide 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS702060 Testing Data
Sequence Length
508
OMIM
609300
3D Structure
ModBase 3D Structure for P05093
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human 17-alpha-OH. No significant cross-reactivity or interference between human 17-alpha-OH and analogues was observed.
Samples
Serum, plasma and tissue homogenates
Assay Type
Competitive
Detection Range
50 pg/ml -2000 pg/ml.
Sensitivity
The minimum detectable dose of human 17-alpha-OH is typically less than 50 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human 17-alpha-OH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP17A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYP17A1purchase
MBS702060 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP17A1. The ELISA analytical biochemical technique of the MBS702060 kit is based on CYP17A1 antibody-CYP17A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP17A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP17A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP17A1 elisa kit
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for 17-alpha-OH and Horseradish Peroxidase (HRP) conjugated 17-alpha-OH. The competitive inhibition reaction is launched between with HRP labeled 17-alpha-OH and unlabeled 17-alpha-OH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of 17-alpha-OH in the sample. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CYP17A1 elisa kit
CYP17A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS702060. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP17A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,371 Da
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450, family 17, subfamily A, polypeptide 1
NCBI Official Symbol
CYP17A1  [Similar Products]
NCBI Official Synonym Symbols
CPT7; CYP17; S17AH; P450C17
  [Similar Products]
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase; CYPXVII; cytochrome P450c17; cytochrome P450-C17; cytochrome P450 17A1; cytochrome p450 XVIIA1; steroid 17-alpha-monooxygenase; 17-alpha-hydroxyprogesterone aldolase; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Synonym Protein Names
17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase
UniProt Gene Name
CYP17A1  [Similar Products]
UniProt Synonym Gene Names
CYP17; S17AH; Cytochrome P450c17  [Similar Products]
UniProt Entry Name
CP17A_HUMAN
NCBI Summary for CYP17A1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP17A1
Function: Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.1 PublicationManual assertion based on experiment in:Ref.10

Catalytic activity: A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.1 PublicationManual assertion based on experiment in:Ref.10

Cofactor: Heme group.1 PublicationManual assertion based on experiment in:Ref.10

Enzyme regulation: Regulated predominantly by intracellular cAMP levels.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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