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CYP24A1 elisa kit :: Mouse 1, 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial (CYP24A1) , cytochrome P450, family 24, subfamily A, polypeptide 1 ELISA Kit

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Catalog # MBS935793
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Typical Testing Data/Standard Curve (for reference only)
Product Name

1, 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial (CYP24A1) , cytochrome P450, family 24, subfamily A, polypeptide 1, ELISA Kit

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Also Known As

Mouse 1, 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial, CYP24A1 ELISA Kit

Product Synonym Names
Mouse 1; 25-dihydroxyvitamin D (3) 24-hydroxylase; mitochondrial (CYP24A1) ELISA kit; CP24; CYP24; MGC126273; MGC126274; P450-CC24; 1; 25-@dihydroxyvitamin D3 24-hydroxylase; 24-OHase; cytochrome P450 family 24 subfamily A polypeptide 1; cytochrome P450; subfamily XXIV (vitamin D 24-hydroxylase) ; exo-mitochondrial; 1; 25-dihydroxyvitamin D (3) 24-hydroxylase; mitochondrial (CYP24A1); cytochrome P450; family 24; subfamily A; polypeptide 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS935793 Testing Data
Sequence Length
514
3D Structure
ModBase 3D Structure for Q64441
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CYP24A1. No significant cross-reactivity or interference between mouse CYP24A1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
3.12 pg/ml-200 pg/ml
Sensitivity
<0.78 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP24A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYP24A1purchase
MBS935793 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1, 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial (CYP24A1), cytochrome P450, family 24, subfamily A, polypeptide 1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP24A1. The ELISA analytical biochemical technique of the MBS935793 kit is based on CYP24A1 antibody-CYP24A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP24A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP24A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Intended use of this ELISA Kit is quantitative determination of mouse mitochondrial 1,25-dihydroxyvitamin D3 24-hydroxylase.

Target protein, CYP24A1, is monooxygenase from cytochrome P450 superfamily of enzymes, and is involved in maintaining calcium homeostasis through regulation of vitamin D metabolism by catalyzing hydroxylation of 1,25-dihydroxyvitamin D3 (calcitriol) side chain.

Test system is designed as sandwich-type ELISA and concentration of CYP24A1is measured using pair of antibodies specific to target protein.

Kit is applicable for testing variable types of samples, including serum, plasma, tissue homogenates and cell lysates.

Product contains microwell plate pre-coated with purified capture antibodies against CYP24A1 and all reagents required for its quantification, including sample and reagent diluents, and wash solution.

Assay kit provides colorimetric detection of CYP24A1 using biotinylated detector antibodies against CYP24A1, avidin-HRP conjugate, and TMB as its substrate solution.

Minimal detectable concentration of ELISA kit is 0.78pg/mL and its linear range is 3.12-200pg/mL; concentrated calibrators for standard curve are provided at two levels and require dilution for preparing working levels.

Intra- and inter-assay CV values, reported as kitÂ’s precision characteristics, are not greater than 8% and 10%, respectively.

Assay doesnÂ’t show significant cross-reactivity with analogous proteins.

ELISA kit and its reagents and components require refrigeration and must be stored at 2-8°C until indicated expiration date.
Related Product Information for
CYP24A1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP24A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP24A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP24A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP24A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CYP24A1 elisa kit
CYP24A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS935793. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP24A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
59,453 Da
NCBI Official Full Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450, family 24, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp24a1  [Similar Products]
NCBI Official Synonym Symbols
CP24; Cyp24; 24-OHase
  [Similar Products]
NCBI Protein Information
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; cytochrome P450, 24; cytochrome P450 24A1; cytochrome P450-CC24; cytochrome P450, 24a1; vitamin D(3) 24-hydroxylase; 25-hydroxyvitamin D-24-hydroxylase
UniProt Protein Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Synonym Protein Names
Cytochrome P450 24A1; Cytochrome P450-CC24
UniProt Gene Name
Cyp24a1  [Similar Products]
UniProt Synonym Gene Names
Cyp-24; Cyp24; 24-OHase; Vitamin D(3) 24-hydroxylase  [Similar Products]
UniProt Entry Name
CP24A_MOUSE
NCBI Summary for CYP24A1
The protein encoded by this gene localizes to the mitochondrion, where it degrades calcitriol to calcitetrol. This gene is upregulated by binding of calcitriol to the upstream regulatory region and to a downstream enhancer region, thereby allowing calcitriol to autoregulate its concentration in the cell. The encoded protein may also play a role in calcium homeostasis. [provided by RefSeq, Aug 2015]
UniProt Comments for CYP24A1
CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 1.14.13.126; Oxidoreductase

Cellular Component: mitochondrion

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; metal ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; monooxygenase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity

Biological Process: response to vitamin D; vitamin D metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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