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CYP27B1 elisa kit :: Chicken 25-hydroxyvitamin D-1 alpha hydroxylase ELISA Kit

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Catalog # MBS2605729
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

25-hydroxyvitamin D-1 alpha hydroxylase (CYP27B1), ELISA Kit

Popular Item
Full Product Name

Mouse 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
No cross-reaction with other factors.
Chicken serum, plasma or cell culture supernatant
Detection Range
20 ng/ml-0.312 ng/ml
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP27B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYP27B1purchase
MBS2605729 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 25-hydroxyvitamin D-1 alpha hydroxylase (CYP27B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP27B1. The ELISA analytical biochemical technique of the MBS2605729 kit is based on CYP27B1 antibody-CYP27B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP27B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP27B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP27B1 elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken CYP27B1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of CYP27B1 elisa kit
CYP27B1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2605729. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP27B1. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,504 Da
NCBI Official Full Name
25-hydroxyvitamin D-1-alpha-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450 family 27 subfamily B member 1
NCBI Official Symbol
CYP27B1  [Similar Products]
NCBI Official Synonym Symbols
VDR; CP2B; CYP1; PDDR; VDD1; VDDR; VDDRI; CYP27B; P450c1; CYP1alpha
  [Similar Products]
NCBI Protein Information
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
UniProt Protein Name
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
UniProt Synonym Protein Names
25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1
UniProt Gene Name
CYP27B1  [Similar Products]
UniProt Synonym Gene Names
CYP1ALPHA; CYP27B; VD3 1A hydroxylase  [Similar Products]
UniProt Entry Name
NCBI Summary for CYP27B1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP27B1
CYP27B1: Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. Defects in CYP27B1 are the cause of rickets vitamin D- dependent type 1A (VDDR1A); also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25- dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - steroid biosynthesis; Mitochondrial; Cell cycle regulation; EC; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: cytoplasm; mitochondrial outer membrane; mitochondrion

Molecular Function: calcidiol 1-monooxygenase activity; heme binding; iron ion binding

Biological Process: bone mineralization; calcium ion homeostasis; calcium ion transport; decidualization; fat-soluble vitamin metabolic process; negative regulation of cell growth; negative regulation of cell proliferation; positive regulation of keratinocyte differentiation; regulation of bone mineralization; response to estrogen stimulus; response to lipopolysaccharide; response to vitamin D; vitamin D catabolic process; vitamin D metabolic process; vitamin metabolic process; xenobiotic metabolic process

Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1a
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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