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CYP2E1 elisa kit :: Mouse cytochrome P450, family 2, subfamily E, polypeptide 1 ELISA Kit

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Catalog # MBS933878
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Typical Testing Data/Standard Curve (for reference only)
Product Name

cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), ELISA Kit

Popular Item
Full Product Name

Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

Product Synonym Names
Mouse Cytochrome P450 2E1 (CYP2E1) ELISA kit; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; cytochrome P450; subfamily IIE (ethanol-inducible); polypeptide 1; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenas; cytochrome P450; family 2; subfamily E; polypeptide 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS933878 Testing Data
3D Structure
ModBase 3D Structure for Q05421
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CYP2E1. No significant cross-reactivity or interference between mouse CYP2E1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
39 pg/ml-2500 pg/ml.
Sensitivity
9.75 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP2E1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CYP2E1purchase
MBS933878 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP2E1. The ELISA analytical biochemical technique of the MBS933878 kit is based on CYP2E1 antibody-CYP2E1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP2E1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP2E1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP2E1 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP2E1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP2E1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2E1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2E1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CYP2E1 elisa kit
CYP2E1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS933878. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP2E1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,805 Da
NCBI Official Full Name
cytochrome P450 2E1
NCBI Official Synonym Full Names
cytochrome P450, family 2, subfamily e, polypeptide 1
NCBI Official Symbol
Cyp2e1  [Similar Products]
NCBI Official Synonym Symbols
Cyp2e
  [Similar Products]
NCBI Protein Information
cytochrome P450 2E1; CYPIIE1; cytochrome P450-J; cytochrome P450-ALC; 4-nitrophenol 2-hydroxylase; cytochrome P450, 2e1, ethanol inducible
UniProt Protein Name
Cytochrome P450 2E1
UniProt Synonym Protein Names
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-ALC; Cytochrome P450-J
Protein Family
UniProt Gene Name
Cyp2e1  [Similar Products]
UniProt Synonym Gene Names
Cyp2e; Cyp2e-1  [Similar Products]
UniProt Entry Name
CP2E1_MOUSE
UniProt Comments for CYP2E1
CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.n7; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; Oxidoreductase; Mitochondrial

Cellular Component: Golgi membrane; intrinsic to endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; mitochondrion; endoplasmic reticulum

Molecular Function: 3-(3-hydroxy-phenyl)propionate hydroxylase activity; toluene-sulfonate methyl-monooxygenase activity; xylene monooxygenase activity; chlorophenol 4-monooxygenase activity; 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; metal ion binding; oxidoreductase activity; carbon disulfide oxygenase activity; toluene 4-monooxygenase activity; phenanthrene 9,10-monooxygenase activity; 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity; 2-hydroxy-phenylacetate hydroxylase activity; 4-chlorobenzaldehyde oxidase activity; enzyme binding; 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; limonene monooxygenase activity; dibenzothiophene monooxygenase activity; phenanthrene 1,2-monooxygenase activity; 3,5-xylenol methylhydroxylase activity; 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; styrene monooxygenase activity; toluene 3-monooxygenase activity; monooxygenase activity; dimethyl sulfide monooxygenase activity; 4-hydroxyphenylacetate 3-monooxygenase activity; 4-nitrophenol 4-monooxygenase activity; 1-hydroxy-2-naphthoate hydroxylase activity; toluene-4-sulfonate monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; tetrahydrofuran hydroxylase activity; oxygen binding; phenylacetate hydroxylase activity; toluene 2-monooxygenase activity; iron ion binding; 6-hydroxynicotinate 3-monooxygenase activity; heme binding; phenanthrene 3,4-monooxygenase activity; arachidonic acid epoxygenase activity; alpha-pinene monooxygenase [NADH] activity; steroid hydroxylase activity; 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Biological Process: steroid metabolic process; triacylglycerol metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; drug metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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