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CYP2E1 elisa kit :: Rat Cytochrome P450 2E1 (CYP2E1) ELISA Kit

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Catalog # MBS2023931 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $265 +1 FREE 8GB USB
  48-Strip-Wells  /  $490 +1 FREE 8GB USB
  96-Strip-Wells  /  $655 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,660 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,795 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Cytochrome P450 2E1 (CYP2E1), ELISA Kit

Popular Item
Also Known As

Cytochrome P450 2E1 (CYP2E1) ELISA Kit

Product Synonym Names
CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
493
OMIM
124040
3D Structure
ModBase 3D Structure for P05181
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 2E1 (CYP2E1).
No significant cross-reactivity or interference between Cytochrome P450 2E1 (CYP2E1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.064ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Cytochrome P450 2E1 (CYP2E1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Cytochrome P450 2E1 (CYP2E1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP2E1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYP2E1purchase
MBS2023931 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 2E1 (CYP2E1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP2E1. The ELISA analytical biochemical technique of the MBS2023931 kit is based on CYP2E1 antibody-CYP2E1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP2E1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP2E1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP2E1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1 (CYP2E1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2E1 (CYP2E1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2E1 (CYP2E1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2E1 (CYP2E1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CYP2E1 elisa kit

CYP2E1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2023931. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP2E1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,849 Da
NCBI Official Full Name
cytochrome P450 2E1
NCBI Official Synonym Full Names
cytochrome P450, family 2, subfamily E, polypeptide 1
NCBI Official Symbol
CYP2E1  [Similar Products]
NCBI Official Synonym Symbols
CPE1; CYP2E; P450-J; P450C2E
  [Similar Products]
NCBI Protein Information
cytochrome P450 2E1
UniProt Protein Name
Cytochrome P450 2E1
UniProt Synonym Protein Names
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-J
Protein Family
UniProt Gene Name
CYP2E1  [Similar Products]
UniProt Synonym Gene Names
CYP2E  [Similar Products]
UniProt Entry Name
CP2E1_HUMAN
NCBI Summary for CYP2E1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP2E1
CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.n7; Mitochondrial; Lipid Metabolism - linoleic acid; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: intrinsic to endoplasmic reticulum membrane; Golgi membrane; endoplasmic reticulum membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; oxidoreductase activity; steroid hydroxylase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; response to ethanol; triacylglycerol metabolic process; response to ozone; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; response to organic nitrogen; drug metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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