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CYP3A4 elisa kit :: Human cytochrome P450, family 3, subfamily A, polypeptide 4 ELISA Kit

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Catalog # MBS934129
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Typical Testing Data/Standard Curve (for reference only)
Product Name

cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), ELISA Kit

Full Product Name

Human Cytochrome P450 3A4, CYP3A4 ELISA Kit

Product Synonym Names
Human Cytochrome P450 3A4 (CYP3A4) ELISA kit; CP33; CP34; CYP3A; CYP3A3; HLP; MGC126680; NF-25; P450C3; P450PCN1; P450-III; steroid inducible; cytochrome P450; subfamily IIIA (niphedipine oxidase); polypeptide 3; cytochrome P450; subfamily IIIA (; cytochrome P450; family 3; subfamily A; polypeptide 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS934129 Testing Data
OMIM
124010
3D Structure
ModBase 3D Structure for P08684
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CYP3A4. No significant cross-reactivity or interference between Human CYP3A4 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
23.5 pg/ml-1500 pg/ml
Sensitivity
5.8 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP3A4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CYP3A4purchase
MBS934129 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP3A4. The ELISA analytical biochemical technique of the MBS934129 kit is based on CYP3A4 antibody-CYP3A4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP3A4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP3A4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP3A4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP3A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP3A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP3A4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP3A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CYP3A4 elisa kit
CYP3A4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS934129. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP3A4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,343 Da
NCBI Official Full Name
cytochrome P450 3A4 isoform 2
NCBI Official Synonym Full Names
cytochrome P450, family 3, subfamily A, polypeptide 4
NCBI Official Symbol
CYP3A4  [Similar Products]
NCBI Official Synonym Symbols
HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
  [Similar Products]
NCBI Protein Information
cytochrome P450 3A4; nifedipine oxidase; cytochrome P450 3A3; cytochrome P450 HLp; cytochrome P450-PCN1; cytochrome P450 NF-25; albendazole sulfoxidase; quinine 3-monooxygenase; albendazole monooxygenase; P450-III, steroid inducible; glucocorticoid-inducible P450; 1,8-cineole 2-exo-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
UniProt Protein Name
Cytochrome P450 3A4
UniProt Synonym Protein Names
1,8-cineole 2-exo-monooxygenase (EC:1.14.13.157); Albendazole monooxygenase (EC:1.14.13.32); Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase (EC:1.14.13.67); Taurochenodeoxycholate 6-alpha-hydroxylase (EC:1.14.13.97)
Protein Family
UniProt Gene Name
CYP3A4  [Similar Products]
UniProt Synonym Gene Names
CYP3A3  [Similar Products]
UniProt Entry Name
CP3A4_HUMAN
NCBI Summary for CYP3A4
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]
UniProt Comments for CYP3A4
CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cell surface; Membrane protein, integral; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.14.14.1; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm; integral to membrane

Molecular Function: quinine 3-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; albendazole monooxygenase activity; taurochenodeoxycholate 6alpha-hydroxylase activity; oxidoreductase activity; oxygen binding; vitamin D3 25-hydroxylase activity; steroid binding; testosterone 6-beta-hydroxylase activity; enzyme binding; iron ion binding; heme binding; steroid hydroxylase activity; monooxygenase activity

Biological Process: steroid metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; androgen metabolic process; monoterpenoid metabolic process; alkaloid catabolic process; lipid metabolic process; heterocycle metabolic process; drug metabolic process; steroid catabolic process; vitamin D metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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