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CYP7A1 elisa kit :: Mouse cytochrome P450, family 7, subfamily A, polypeptide 1 ELISA Kit

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Catalog # MBS922713
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), ELISA Kit

Also Known As

Mouse Cholesterol 7-alpha-monooxygenase, CYP7A1 ELISA Kit

Product Synonym Names
Mouse Cholesterol 7-alpha-monooxygenase (CYP7A1) ELISA kit; CP7A; CYP7; MGC126826; MGC138389; cholesterol 7 alpha-monooxygenase; cholesterol 7-alpha-hydroxylase; cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1; cytochrome P450; family 7; subfamily A; polypeptide 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS922713 Testing Data
Sequence Length
503
3D Structure
ModBase 3D Structure for Q64505
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CYP7A1. No significant cross-reactivity or interference between mouse CYP7A1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
47 pg/ml -3000 pg/ml.
Sensitivity
The minimum detectable dose of mouse CYP7A1 is typically less than 11.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYP7A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCYP7A1purchase
MBS922713 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP7A1. The ELISA analytical biochemical technique of the MBS922713 kit is based on CYP7A1 antibody-CYP7A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP7A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP7A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYP7A1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP7A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP7A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of CYP7A1 elisa kit
CYP7A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS922713. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYP7A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,262 Da
NCBI Official Full Name
cholesterol 7-alpha-monooxygenase
NCBI Official Synonym Full Names
cytochrome P450, family 7, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp7a1  [Similar Products]
NCBI Protein Information
cholesterol 7-alpha-monooxygenase; CYPVII; cytochrome P450 7A1; cytochrome P450, 7a1; cholesterol 7 alpha hydroxylase; cholesterol 7-alpha-hydroxylase
UniProt Protein Name
Cholesterol 7-alpha-monooxygenase
UniProt Synonym Protein Names
CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1
UniProt Gene Name
Cyp7a1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CP7A1_MOUSE
UniProt Comments for CYP7A1
CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; Endoplasmic reticulum; EC 1.14.13.17

Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; cholesterol 7-alpha-monooxygenase activity; metal ion binding; iron ion binding; heme binding; oxidoreductase activity; monooxygenase activity

Biological Process: steroid metabolic process; cholesterol metabolic process; bile acid biosynthetic process; cholesterol homeostasis; lipid metabolic process; cholesterol catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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