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CYSLTR1 elisa kit :: Guinea Pig CYSLTR1 ELISA Kit

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Catalog # MBS2511358
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Typical Testing Data/Standard Curve (for reference only)
Product Name

CYSLTR1, ELISA Kit

Full Product Name

Guinea pig CYSLTR1 (Cysteinyl Leukotriene Receptor 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
300201
3D Structure
ModBase 3D Structure for Q9Y271
Species Reactivity
Specificity
This kit recognizes natural and recombinant Guinea pig CYSLTR1. No significant cross-reactivity or interference between Guinea pig CYSLTR1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CYSLTR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CYSLTR1purchase
MBS2511358 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CYSLTR1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYSLTR1. The ELISA analytical biochemical technique of the MBS2511358 kit is based on CYSLTR1 antibody-CYSLTR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYSLTR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYSLTR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
CYSLTR1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig CYSLTR1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CYSLTR1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CYSLTR1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CYSLTR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CYSLTR1. You can calculate the concentration of CYSLTR1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of CYSLTR1 elisa kit
CYSLTR1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2511358. Click to request current manual
NCBI/Uniprot data below describe general gene information for CYSLTR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,541 Da
NCBI Official Full Name
cysteinyl leukotriene receptor 1
NCBI Official Synonym Full Names
cysteinyl leukotriene receptor 1
NCBI Official Symbol
CYSLTR1  [Similar Products]
NCBI Official Synonym Symbols
CYSLT1; CYSLTR; CYSLT1R; HMTMF81
  [Similar Products]
NCBI Protein Information
cysteinyl leukotriene receptor 1; G-protein coupled receptor HG55; LTD4 receptor; cysteinyl leukotriene D4 receptor
UniProt Protein Name
Cysteinyl leukotriene receptor 1
UniProt Synonym Protein Names
Cysteinyl leukotriene D4 receptor; LTD4 receptor; G-protein coupled receptor HG55; HMTMF81
UniProt Gene Name
CYSLTR1  [Similar Products]
UniProt Synonym Gene Names
CYSLT1; CysLTR1; LTD4 receptor  [Similar Products]
UniProt Entry Name
CLTR1_HUMAN
NCBI Summary for CYSLTR1
This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for cysteinyl leukotrienes, and is involved in mediating bronchoconstriction via activation of a phosphatidylinositol-calcium second messenger system. Activation of the encoded receptor results in contraction and proliferation of bronchial smooth muscle cells, eosinophil migration, and damage to the mucus layer in the lung. Upregulation of this gene is associated with asthma and dysregulation may also be implicated in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Comments for CYSLTR1
CYSLTR1: Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: Xq13.2-q21.1

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; leukotriene receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; calcium ion transport; positive regulation of vasoconstriction; defense response; respiratory gaseous exchange; chemotaxis; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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