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D2R/DRD2 elisa kit :: Human Dopamine Receptor D2 ELISA Kit

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Catalog # MBS760868
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Dopamine Receptor D2 (D2R/DRD2), ELISA Kit

Popular Item
Full Product Name

Human Dopamine Receptor D2 ELISA Kit

Product Synonym Names
D2R/DRD2 (Dopamine Receptor D2)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS760868 COA
OMIM
103780
3D Structure
ModBase 3D Structure for P14416
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of D2R/DRD2. No significant cross-reactivity or interference between D2R/DRD2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.313-20ng/ml
Sensitivity
<0.188ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D2R/DRD2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D2R/DRD2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of D2R/DRD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forD2R/DRD2purchase
MBS760868 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dopamine Receptor D2 (D2R/DRD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing D2R/DRD2. The ELISA analytical biochemical technique of the MBS760868 kit is based on D2R/DRD2 antibody-D2R/DRD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect D2R/DRD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, D2R/DRD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
D2R/DRD2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- D2R/DRD2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- D2R/DRD2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the D2R/DRD2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of D2R/DRD2 can be calculated.

Typical Testing Data/Standard Curve (for reference only) of D2R/DRD2 elisa kit
D2R/DRD2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS760868. Click to request current manual
NCBI/Uniprot data below describe general gene information for D2R/DRD2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,847 Da
NCBI Official Full Name
dopamine receptor D2
NCBI Official Synonym Full Names
dopamine receptor D2
NCBI Official Symbol
NCBI Official Synonym Symbols
D2R; D2DR
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NCBI Protein Information
D(2) dopamine receptor
UniProt Protein Name
D(2) dopamine receptor
UniProt Synonym Protein Names
Dopamine D2 receptor
UniProt Gene Name
UniProt Entry Name
DRD2_HUMAN
NCBI Summary for D2R/DRD2
This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
UniProt Comments for D2R/DRD2
DRD2: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11); also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: acrosome; axon; cytosol; dendrite; dendritic spine; endocytic vesicle; integral to plasma membrane; intracellular; lateral plasma membrane; nerve terminal; nonmotile primary cilium; perikaryon; plasma membrane; postsynaptic density; synaptic vesicle membrane

Molecular Function: adrenoceptor activity; dopamine binding; dopamine D2 receptor-like receptor activity; drug binding; identical protein binding; ionotropic glutamate receptor binding; potassium channel regulator activity; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: activation of protein kinase activity; adenohypophysis development; adult walking behavior; arachidonic acid secretion; associative learning; auditory behavior; axonogenesis; behavioral response to cocaine; behavioral response to ethanol; branching morphogenesis of a nerve; cellular calcium ion homeostasis; cerebral cortex GABAergic interneuron migration; circadian regulation of gene expression; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; dopamine receptor, phospholipase C activating pathway; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; G-protein coupled receptor internalization; grooming behavior; locomotory behavior; long-term memory; negative regulation of adenylate cyclase activity; negative regulation of blood pressure; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of circadian sleep/wake cycle, sleep; negative regulation of dopamine receptor signaling pathway; negative regulation of dopamine secretion; negative regulation of innate immune response; negative regulation of insulin secretion; negative regulation of protein kinase B signaling cascade; negative regulation of protein secretion; negative regulation of synaptic transmission, glutamatergic; nerve-nerve synaptic transmission; orbitofrontal cortex development; peristalsis; phosphatidylinositol metabolic process; pigmentation; positive regulation of cytokinesis; positive regulation of dopamine uptake; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of growth hormone secretion; positive regulation of multicellular organism growth; positive regulation of neuroblast proliferation; positive regulation of receptor internalization; positive regulation of transcription from RNA polymerase II promoter; prepulse inhibition; protein localization; reduction of cytosolic calcium ion concentration; regulation of cAMP metabolic process; regulation of dopamine secretion; regulation of dopamine uptake; regulation of heart rate; regulation of long-term neuronal synaptic plasticity; regulation of phosphoprotein phosphatase activity; regulation of potassium ion transport; regulation of sodium ion transport; regulation of synapse structural plasticity; regulation of synaptic transmission, GABAergic; regulation of systemic arterial blood pressure by neurological process; release of sequestered calcium ion into cytosol; response to amphetamine; response to axon injury; response to cocaine; response to drug; response to hypoxia; response to inactivity; response to iron ion; response to light stimulus; response to morphine; response to nicotine; response to toxin; sensory perception of smell; striatum development; synaptic transmission, dopaminergic; synaptogenesis; thermoregulation; visual learning; Wnt receptor signaling pathway

Disease: Myoclonic Dystonia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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