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DAD1 recombinant protein :: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Recombinant Protein

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Catalog # MBS717181
Unit / Price
Scan QR to view Datasheet
  0.05 mg (E-Coli)  /  $190 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $460 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $750 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,180 +1 FREE 8GB USB
SDS-Page
Product Name

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Recombinant Protein

Full Product Name

Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1

Product Synonym Names
Defender against cell death 1; DAD-1
Matching Pairs
Antibody: DAD1 (MBS7041818)
Standard: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (MBS717181)
Comments: Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-113
Sequence
SASVVSVISR FLEEYLSSTP QRLKLLDAYL LYILLTGALQ FGYCLLVGTF PFNSFLSGFI SCVGSFILAV CLRIQINPQN KADFQGISPE RAFADFLFAS TILHLVVMNF VG
OMIM
600243
3D Structure
ModBase 3D Structure for P61803
Host
E Coli
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of DAD1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DAD1 recombinant protein
Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
Product Categories/Family for DAD1 recombinant protein

DAD1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for DAD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39.8kD
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
NCBI Official Synonym Full Names
defender against cell death 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
UniProt Synonym Protein Names
Defender against cell death 1; DAD-1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Oligosaccharyl transferase subunit DAD1; DAD-1  [Similar Products]
UniProt Entry Name
DAD1_HUMAN
NCBI Summary for DAD1
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]
UniProt Comments for DAD1
DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family.

Protein type: EC 2.4.99.18; Membrane protein, multi-pass; Glycan Metabolism - N-glycan biosynthesis; Endoplasmic reticulum; Apoptosis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane; membrane; oligosaccharyl transferase complex

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity

Biological Process: apoptosis; blastocyst development; cellular protein metabolic process; negative regulation of apoptosis; post-translational protein modification; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; response to drug; response to nutrient
Product References and Citations for DAD1 recombinant protein
Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.Nakashima T., Sekiguchi T., Kuraoka A., Fukushima K., Shibata Y., Komiyama S., Nishimoto T.Mol. Cell. Biol. 13:6367-6374(1993)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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