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DAO elisa kit :: Human Diamine Oxidase ELISA Kit

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Catalog # MBS722064
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Diamine Oxidase (DAO), ELISA Kit

Full Product Name

Human Diamine Oxidase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
104610
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Highest Standards
50ng/mL
Concentration of Standards
Concentration of Standards
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DAO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDAOpurchase
MBS722064 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Diamine Oxidase (DAO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DAO. The ELISA analytical biochemical technique of the MBS722064 kit is based on DAO antibody-DAO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DAO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DAO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Typical Testing Data/Standard Curve (for reference only) of DAO elisa kit
DAO elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS722064. Click to request current manual
NCBI/Uniprot data below describe general gene information for DAO. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
87,239 Da
NCBI Official Full Name
amiloride-sensitive amine oxidase
NCBI Official Synonym Full Names
amine oxidase, copper containing 1
NCBI Official Symbol
NCBI Official Synonym Symbols
ABP; DAO; KAO; ABP1; DAO1
  [Similar Products]
NCBI Protein Information
amiloride-sensitive amine oxidase [copper-containing]; amiloride-sensitive amine oxidase [copper-containing]; histaminase; diamine oxidase; kidney amine oxidase; amiloride-binding protein 1; amine oxidase copper domain-containing protein 1; amiloride binding protein 1 (amine oxidase (copper-containing))
UniProt Protein Name
Amiloride-sensitive amine oxidase [copper-containing]
UniProt Synonym Protein Names
Amiloride-binding protein 1; Amine oxidase copper domain-containing protein 1; Histaminase; Kidney amine oxidase; KAO
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ABP1; DAO1; DAO; Diamine oxidase; KAO  [Similar Products]
UniProt Entry Name
AOC1_HUMAN
NCBI Summary for DAO
This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
UniProt Comments for DAO
ABP1: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Belongs to the copper/topaquinone oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Amino Acid Metabolism - histidine; EC 1.4.3.22; Amino Acid Metabolism - arginine and proline; Secreted, signal peptide; Secreted; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: extracellular space; tight junction; plasma membrane; peroxisome

Molecular Function: heparin binding; amine oxidase activity; copper ion binding; protein homodimerization activity; zinc ion binding; protein complex binding; drug binding; cation channel activity; receptor activity; calcium ion binding; sodium channel activity; quinone binding

Biological Process: response to drug; response to antibiotic; amine metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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