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DAT elisa kit :: Monkey Dopamine Transporter ELISA Kit

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Catalog # MBS017068
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DAT elisa kit
Product Name

Dopamine Transporter (DAT), ELISA Kit

Popular Item
Full Product Name

Monkey Dopamine Transporter ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 5; NC_000005.9 (1392905..1445543, complement). Location: 5p15.3
OMIM
126455
3D Structure
ModBase 3D Structure for Q01959
Species Reactivity
Specificity
No significant cross-reactivity or interference between Monkey DAT and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
1.56 ng/ml - 50 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DAT concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey DAT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DAT and DAT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DATpurchase
MBS017068 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dopamine Transporter (DAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DAT. The ELISA analytical biochemical technique of the MBS017068 kit is based on DAT antibody-DAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DAT elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DAT concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey DAT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DAT and DAT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS017068. Click to request current manual
NCBI/Uniprot data below describe general gene information for DAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
68,495 Da
NCBI Official Full Name
dopamine transporter
NCBI Official Synonym Full Names
solute carrier family 6 (neurotransmitter transporter), member 3
NCBI Official Symbol
SLC6A3  [Similar Products]
NCBI Official Synonym Symbols
DAT; DAT1; PKDYS
  [Similar Products]
NCBI Protein Information
sodium-dependent dopamine transporter; DA transporter; dopamine transporter 1; solute carrier family 6 member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
UniProt Protein Name
Sodium-dependent dopamine transporter
UniProt Synonym Protein Names
Solute carrier family 6 member 3
UniProt Gene Name
SLC6A3  [Similar Products]
UniProt Synonym Gene Names
DAT1; DA transporter; DAT  [Similar Products]
UniProt Entry Name
SC6A3_HUMAN
NCBI Summary for DAT
This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]
UniProt Comments for DAT
DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: cell soma; integral to plasma membrane; axon; cytoplasm; plasma membrane; integral to membrane

Molecular Function: protein binding; protease binding; protein complex binding; dopamine binding; monoamine transmembrane transporter activity; protein N-terminus binding; drug binding; dopamine:sodium symporter activity; dopamine transmembrane transporter activity; receptor binding

Biological Process: response to drug; response to nicotine; lactation; dopamine transport; response to cAMP; prepulse inhibition; dopamine catabolic process; regulation of dopamine metabolic process; monoamine transport; sensory perception of smell; locomotory behavior; dopamine biosynthetic process; positive regulation of multicellular organism growth; response to cocaine; synaptic transmission; response to ethanol; neurotransmitter transport; adenohypophysis development; response to iron ion; transmembrane transport; neurotransmitter biosynthetic process; aging

Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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