|
| Product Name
|
|
HTT / Huntingtin, Polyclonal Antibody ★Popular Item★
|
| Also Known As
|
|
Rabbit Polyclonal to Human HTT / Huntingtin
|
| Product Synonym Names
|
|
Anti-HTT / Huntingtin Antibody (phospho-Ser421) IHC-plus; HTT; Huntingtin; Huntington disease protein; IT15; HD; HD protein; Human HTT; Huntingtin
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
|
| OMIM
|
|
phenotype 613004
|
| 3D Structure
|
|
ModBase 3D Structure for P42858
|
|
| Clonality
|
|
Polyclonal
|
| Host
|
|
Rabbit
|
| Species Reactivity
|
|
Human
|
|
|
| Specificity
|
|
Amino acids 416 to 424 of Human huntingtin protein phosphorylated at Ser421
|
| Purity/Purification
|
|
Immunoaffinity Purified
|
| Form/Format
|
|
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
|
| Concentration
|
|
1 mg/ml (lot specific)
|
|
|
| Target Species
|
|
Human
|
| Immunogen Description
|
|
Synthetic peptide from human HTT / Huntingtin.
|
| Immunogen Type
|
|
Synthetic peptide
|
| Immunogen
|
|
HTT / Huntingtin antibody was raised against synthetic peptide from human HTT / Huntingtin.
|
| Antigen Modification
|
|
pSer421
|
|
|
| Preparation and Storage
|
|
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
|
| Other Notes
|
|
Small volumes of anti-HTT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
|
TOP
|
|
|
|
|
Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological.
|
| Product Categories/Family for anti-HTT antibody
|
|
Family: Hungtintin
|
|
| Applications Tested/Suitable for anti-HTT antibody
|
|
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
|
| Application Notes for anti-HTT antibody
|
|
ELISA (1:10000 - 1:40000), IHC-P (10 - 20 ug/ml), WB (1:500 - 1:3000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
|
|
|
| Immunohistochemistry (IHC) of anti-HTT antibody
|
|
Anti-HTT / Huntingtin antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
|
|
|
| Western Blot (WB) of anti-HTT antibody
|
|
Anti-Huntingtin pS421 Antibody - Western Blot. Western blot analysis after AKT and phosphatase treatment is shown using Affinity Purified anti-Huntingtin pS421 antibody. In A) untreated lysates from N2A and 293A cells were stained directly using anti-Huntingtin pS421 antibody. Full length staining of Huntingtin is noted, albeit at low levels of expression, as well as a strongly staining band at 200 kD that may represent staining of truncated protein. In B) staining is shown after immunoprecipitation using a monoclonal antibody (Mab2166) followed by AKT treatment (to phosphorylate), along with untreated, and phosphatase (PP) treated (dephosphorylate) immunoprecipitated. Full length phosphorylated huntingtin is clearly detected in these immunopurified samples (except dephosphorylated). In C) lysates are treated directly with AKT or PP to alter the phosphorylation status of Htt. Personal communication, Simon Warby, CMMT, Vancouver, BC.
|
|
|
| Western Blot (WB) of anti-HTT antibody
|
|
Anti-Huntingtin pS421 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Huntingtin pS421 antibody to detect endogenous protein present in an unstimulated human PC-3 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~190 kD corresponding to truncated human Huntingtin protein. The blot was incubated with a 1:1000 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication,.
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for HTT. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
90903231
|
| NCBI GeneID
|
|
3064
|
| NCBI Accession #
|
|
NP_002102.4
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_002111.7
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
P42858
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
Q9UQB7
|
[Other Products]
|
| UniProt Related Accession #
|
|
P42858
|
[Other Products]
|
| Molecular Weight
|
|
347,603 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
huntingtin
|
| NCBI Official Synonym Full Names
|
|
huntingtin
|
| NCBI Official Symbol
|
|
HTT
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
HD; IT15
|
[Similar Products]
|
| NCBI Protein Information
|
|
huntingtin; huntington disease protein
|
| UniProt Protein Name
|
|
Huntingtin
|
| UniProt Synonym Protein Names
|
|
Huntington disease protein; HD protein
|
| Protein Family
|
|
HD protein
|
| UniProt Gene Name
|
|
HTT
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
HD; IT15; HD protein
|
[Similar Products]
|
| UniProt Entry Name
|
|
HD_HUMAN
|
|
|
| NCBI Summary for HTT
|
|
Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2008]
|
|
|
| UniProt Comments for HTT
|
|
Huntingtin: may play a role in microtubule-mediated transport or vesicle function. Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Defects are the cause of Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the Huntingtin gene, which translates as a polyglutamine repeat in the protein product. The Huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The Huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated isoforms displaying different relative abundance in various fetal and adult tissues.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 4p16.3
Cellular Component: Golgi apparatus; cytoplasmic vesicle membrane; protein complex; mitochondrion; endoplasmic reticulum; dendrite; autophagic vacuole; inclusion body; cytosol; nucleoplasm; axon; cytoplasm; late endosome; nucleus
Molecular Function: identical protein binding; protein binding; p53 binding; beta-tubulin binding; dynein intermediate chain binding; diazepam binding; transcription factor binding
Biological Process: ER to Golgi vesicle-mediated transport; citrulline metabolic process; paraxial mesoderm formation; regulation of protein phosphatase type 2A activity; regulation of synaptic plasticity; locomotory behavior; determination of adult life span; endosome transport; anterior/posterior pattern formation; L-glutamate import; regulation of mitochondrial membrane potential; establishment of mitotic spindle orientation; protein import into nucleus; quinolinate biosynthetic process; organ development; retrograde vesicle-mediated transport, Golgi to ER; vesicle transport along microtubule; visual learning; negative regulation of neuron apoptosis; Golgi organization and biogenesis; grooming behavior; endoplasmic reticulum organization and biogenesis; positive regulation of inositol-1,4,5-triphosphate receptor activity; striatum development; axon cargo transport; cell aging; olfactory lobe development; social behavior; lactate biosynthetic process from pyruvate; iron ion homeostasis; neuron apoptosis; insulin secretion; dopamine receptor signaling pathway; neuron development; hormone metabolic process; spermatogenesis; regulation of mitochondrial membrane permeability; response to calcium ion; neural plate formation; urea cycle
Disease: Huntington Disease
|
|
TOP
|
|
| Research Articles on HTT
|
|
1. Findings indicate that the presence of wild-type huntingtin has a beneficial role even when its relative expression level is lower than that of the mutant protein
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with anti-HTT antibody | Pathways associated with anti-HTT antibody |
|
|
|
| Diseases associated with anti-HTT antibody | Organs/Tissues associated with anti-HTT antibody |
|
|
|
|
|
|
TOP
|
Antibody 
