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| Product Name
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eNOS, Antibody ★Popular Item★
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| Also Known As
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eNOS (Phospho-Ser615) Antibody
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| Product Synonym Names
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Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; NOS; type III; NOS3; NOSIII; Nitric-oxide synthase; endothelial; cNOS
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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163729
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| 3D Structure
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ModBase 3D Structure for P29474
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse, Rat
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| Specificity
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eNOS (Phospho-Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.
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| Purity/Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
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| Form/Format
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Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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| Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 615 (F-N-SP-I-S).
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| All Sites
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Human: Ser615; Mouse: Ser614; Rat: Ser614
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| Pathway
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Phospho-specific
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| Preparation and Storage
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Store at -20 degree C for 1 year
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| Other Notes
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Small volumes of anti-eNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Product Categories/Family for anti-eNOS antibody
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Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
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| Applications Tested/Suitable for anti-eNOS antibody
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Western Blot (WB), ELISA (EIA)
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| Application Notes for anti-eNOS antibody
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WB: 1:500~1:1000, ELISA: 1:40000
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| Testing Data of anti-eNOS antibody
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Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.
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| NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product. |
| NCBI GI #
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40254422
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| NCBI GeneID
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4846
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| NCBI Accession #
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NP_000594.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_000603.4
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[Other Products]
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| UniProt Primary Accession #
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P29474
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[Other Products]
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| UniProt Secondary Accession #
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Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2
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[Other Products]
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| UniProt Related Accession #
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P29474
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[Other Products]
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| Molecular Weight
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67,876 Da
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TOP
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| NCBI Official Full Name
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nitric oxide synthase, endothelial isoform 1
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| NCBI Official Synonym Full Names
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nitric oxide synthase 3 (endothelial cell)
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| NCBI Official Symbol
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NOS3
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[Similar Products]
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| NCBI Official Synonym Symbols
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eNOS; ECNOS
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[Similar Products]
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| NCBI Protein Information
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nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21
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| UniProt Protein Name
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Nitric oxide synthase, endothelial
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| UniProt Synonym Protein Names
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Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
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| UniProt Gene Name
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NOS3
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[Similar Products]
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| UniProt Synonym Gene Names
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cNOS; eNOS; NOSIII
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[Similar Products]
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| UniProt Entry Name
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NOS3_HUMAN
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| NCBI Summary for eNOS
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Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
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| UniProt Comments for eNOS
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eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol
Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding
Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; regulation of blood pressure; negative regulation of hydrolase activity; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; regulation of blood vessel size; nitric oxide biosynthetic process; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia
Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic
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Product References and Citations for anti-eNOS antibody
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Janssens S.P., J. Biol. Chem. 267:14519-14522(1992).
Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
Marsden P.A., FEBS Lett. 307:287-293(1992).
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| Research Articles on eNOS
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1. eNOS gene polymorphism is associated with graft function.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Pathways associated with anti-eNOS antibody | Diseases associated with anti-eNOS antibody |
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| Organs/Tissues associated with anti-eNOS antibody |
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Antibody 
