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| Product Name
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eNOS, Antibody ★Popular Item★
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| Also Known As
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eNOS (Phospho-Ser615) Antibody
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| Product Synonym Names
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Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; NOS; type III; NOS3; NOSIII; Nitric-oxide synthase; endothelial; cNOS
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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163729
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| 3D Structure
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ModBase 3D Structure for P29474
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| Species Reactivity
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Human, Mouse, Rat
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| Specificity
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eNOS (Phospho-Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.
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| Purity/Purification
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Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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| Form/Format
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In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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| Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 615 (F-N-SP-I-S).
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| All Sites
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Human: Ser615
Mouse: Ser614
Rat: Ser614
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| Preparation and Storage
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Store at-20 degree C for 1 year.
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| Other Notes
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Small volumes of anti-eNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Product Categories/Family for anti-eNOS antibody
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Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
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| Applications Tested/Suitable for anti-eNOS antibody
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Western Blot (WB), ELISA (EIA)
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| Application Notes for anti-eNOS antibody
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WB: 1:500~1:1000
ELISA: 1:40000
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| Western Blot (WB) of anti-eNOS antibody
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Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.
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| NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product. |
| NCBI GI #
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40254422
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| NCBI GeneID
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4846
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| NCBI Accession #
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NP_000594.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_000603.4
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[Other Products]
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| UniProt Primary Accession #
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P29474
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[Other Products]
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| UniProt Secondary Accession #
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Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2
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[Other Products]
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| UniProt Related Accession #
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P29474
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[Other Products]
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| Molecular Weight
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67,862 Da
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| NCBI Official Full Name
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nitric oxide synthase, endothelial isoform 1
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| NCBI Official Synonym Full Names
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nitric oxide synthase 3
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| NCBI Official Symbol
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NOS3
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[Similar Products]
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| NCBI Official Synonym Symbols
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eNOS; ECNOS
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[Similar Products]
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| NCBI Protein Information
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nitric oxide synthase, endothelial
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| UniProt Protein Name
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Nitric oxide synthase, endothelial
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| UniProt Synonym Protein Names
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Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
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| UniProt Gene Name
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NOS3
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[Similar Products]
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| UniProt Synonym Gene Names
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cNOS; eNOS; NOSIII
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[Similar Products]
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| NCBI Summary for eNOS
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Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
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| UniProt Comments for eNOS
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Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
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Product References and Citations for anti-eNOS antibody
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Janssens S.P., J. Biol. Chem. 267:14519-14522(1992). Janssens S.P., J. Biol. Chem. 267:22694-22694(1992). Marsden P.A., FEBS Lett. 307:287-293(1992).
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| Research Articles on eNOS
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1. 27-bp VNTR polymorphism in intron 4 of eNOS gene polymorphism may be the significant risk factor for systemic lupus erythematosus in south Indian subjects.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-eNOS antibody | Pathways associated with anti-eNOS antibody |
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| Diseases associated with anti-eNOS antibody | Organs/Tissues associated with anti-eNOS antibody |
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Antibody 
