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MUC5AC elisa kit :: Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS454469 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $345 +1 FREE 8GB USB
96-Strip-Wells  /  $455 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,025 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,920 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mucin 5 Subtype AC (MUC5AC), ELISA Kit

★Popular Item★
 Also Known As   

Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit

 Product Synonym Names    TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
 Product Gene Name   

MUC5AC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    FO680660 Genomic DNA
 3D Structure    ModBase 3D Structure for P98088
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed.
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 Samples    Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    78.125-5000pg/mL
 Sensitivity    28pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUC5AC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUC5AC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MUC5AC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MUC5AC purchase    MBS454469 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mucin 5 Subtype AC (MUC5AC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.125-5000pg/mL in biological research samples containing MUC5AC, with an estimated sensitivity of 28pg/mL. The ELISA analytical biochemical technique of the MBS454469 kit is based on MUC5AC antibody-MUC5AC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC5AC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC5AC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MUC5AC elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MUC5AC in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUC5AC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MUC5AC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUC5AC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUC5AC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MUC5AC elisa kit    MUC5AC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS454469. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MUC5AC. It may not necessarily be applicable to this product.
 NCBI GI #    748983076
 NCBI GeneID    4586
 NCBI Accession #    NP_001291288.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001304359.1 [Other Products]
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 UniProt Primary Accession #    P98088 [Other Products]
 UniProt Secondary Accession #    O60460; O76065; Q13792; Q14425; Q658Q1; Q7M4S5; Q8N4M9; Q8WWQ3; Q8WWQ4; Q8WWQ5; A0A096LPK4 [Other Products]
 UniProt Related Accession #    P98088 [Other Products]
 Molecular Weight    585,570 Da
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 NCBI Official Full Name    mucin-5AC
 NCBI Official Synonym Full Names    mucin 5AC, oligomeric mucus/gel-forming
 NCBI Official Symbol    MUC5AC [Similar Products]
 NCBI Official Synonym Symbols   
TBM; leB; MUC5; mucin
[Similar Products]
 NCBI Protein Information    mucin-5AC
 UniProt Protein Name    Mucin-5AC
 UniProt Synonym Protein Names   
Gastric mucin
 Protein Family    Mucin
 UniProt Gene Name    MUC5AC [Similar Products]
 UniProt Synonym Gene Names    TBM [Similar Products]
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 UniProt Comments for MUC5AC    Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
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 Research Articles on MUC5AC    1. this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with MUC5AC elisa kitDiseases associated with MUC5AC elisa kit
 Products by Pathway  Pathway Diagram
 C-type Lectin Receptors (CLRs) Pathway antibodies  C-type Lectin Receptors (CLRs) Pathway Diagram
 Dectin-2 Family Pathway antibodies  Dectin-2 Family Pathway Diagram
 Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway antibodies  Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway Diagram
 Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway antibodies  Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway Diagram
 Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway antibodies  Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With O-glycosylation Of Proteins Pathway antibodies  Diseases Associated With O-glycosylation Of Proteins Pathway Diagram
 Diseases Of Glycosylation Pathway antibodies  Diseases Of Glycosylation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >415 publications with MUC5AC and Lung Diseases
 Inflammation Antibodies  >396 publications with MUC5AC and Inflammation
 Asthma Antibodies  >276 publications with MUC5AC and Asthma
 Metaplasia Antibodies  >262 publications with MUC5AC and Metaplasia
 Hypersensitivity Antibodies  >177 publications with MUC5AC and Hypersensitivity
 Hyperplasia Antibodies  >172 publications with MUC5AC and Hyperplasia
 Respiratory Hypersensitivity Antibodies  >161 publications with MUC5AC and Respiratory Hypersensitivity
 Necrosis Antibodies  >137 publications with MUC5AC and Necrosis
 Disease Models, Animal Antibodies  >136 publications with MUC5AC and Disease Models, Animal
 Fibrosis Antibodies  >122 publications with MUC5AC and Fibrosis
Organs/Tissues associated with MUC5AC elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >546 publications with MUC5AC and Lung
 Intestine Antibodies  >380 publications with MUC5AC and Intestine
 Stomach Antibodies  >368 publications with MUC5AC and Stomach
 Eye Antibodies  >205 publications with MUC5AC and Eye
 Pancreas Antibodies  >129 publications with MUC5AC and Pancreas
 Mammary Gland Antibodies  >4 publications with MUC5AC and Mammary Gland
 Uncharacterized Tissue Antibodies  >1 publications with MUC5AC and Uncharacterized Tissue
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