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CDH10 elisa kit :: Rat Cadherin-10 (CDH10) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7201919 CDH10 elisa kit
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cadherin-10 (CDH10), ELISA Kit

★Popular Item★
 Also Known As   

Rat Cadherin-10 (CDH10) ELISA Kit

 Product Gene Name   

CDH10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604555
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CDH10. No significant cross-reactivity or interference between CDH10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDH10 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/ml
 Intended Uses    This CDH10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CDH10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDH10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDH10 purchase    MBS7201919 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cadherin-10 (CDH10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing CDH10, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS7201919 kit is based on CDH10 antibody-CDH10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CDH10 elisa kit

   Principle of the Assay: CDH10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDH10 antibody and an CDH10-HRP conjugate. The assay sample and buffer are incubated together with CDH10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDH10 concentration since CDH10 from samples and CDH10-HRP conjugate compete for the anti-CDH10 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDH10 from the sample, fewer sites are left to bind CDH10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDH10 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CDH10 elisa kit    Signal Transduction
Sample Manual Insert of MBS7201919. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CDH10. It may not necessarily be applicable to this product.
 NCBI GI #    16306530
 NCBI GeneID    1008
 NCBI Accession #    NP_006718.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006727.3 [Other Products]
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 UniProt Secondary Accession #    Q9ULB3 [Other Products]
 UniProt Related Accession #    Q9Y6N8 [Other Products]
 Molecular Weight    88,451 Da
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 NCBI Official Full Name    cadherin-10 preproprotein
 NCBI Official Synonym Full Names    cadherin 10, type 2 (T2-cadherin)
 NCBI Official Symbol    CDH10 [Similar Products]
 NCBI Protein Information    cadherin-10; T2-cadherin; cadherin 10 type 2
 UniProt Protein Name    Cadherin-10
 UniProt Synonym Protein Names   
T2-cadherin
 Protein Family    Cadherin
 UniProt Gene Name    CDH10 [Similar Products]
 UniProt Entry Name    CAD10_HUMAN
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 NCBI Summary for CDH10    This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. [provided by RefSeq, Jan 2011]
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 UniProt Comments for CDH10    CDH10: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p14.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; homophilic cell adhesion
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 Research Articles on CDH10    1. The CC genotype of rs4307059 located in the region between CDH9 and CDH10 is associated with completed suicide in a Polish cohort
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDH10 elisa kitPathways associated with CDH10 elisa kit
 Reference Product  PubMed Publications
 CDH9 elisa kit  >5 publications with CDH10 and CDH9
 CDH18 elisa kit  >3 publications with CDH10 and CDH18
 CDH13 elisa kit  >2 publications with CDH10 and CDH13
 CDH5 elisa kit  >2 publications with CDH10 and CDH5
 CDH15 elisa kit  >2 publications with CDH10 and CDH15
 CDH2 elisa kit  >1 publications with CDH10 and CDH2
 CDH4 elisa kit  >1 publications with CDH10 and CDH4
 CDH6 elisa kit  >1 publications with CDH10 and CDH6
 Products by Pathway  Pathway Diagram
 Adherens Junctions Interactions Pathway antibodies  Adherens Junctions Interactions Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
Diseases associated with CDH10 elisa kitOrgans/Tissues associated with CDH10 elisa kit
 Disease Name  Pubmed Publications
 Hyperplasia Antibodies  >1 publications with CDH10 and Hyperplasia
 Autistic Disorder Antibodies  >1 publications with CDH10 and Autistic Disorder
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with CDH10 and Brain
 Lung Antibodies  >3 publications with CDH10 and Lung
 Embryonic Tissue Antibodies  >2 publications with CDH10 and Embryonic Tissue
 Mixed Antibodies  >1 publications with CDH10 and Mixed
 Muscle Antibodies  >1 publications with CDH10 and Muscle
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