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anti-DAXX antibody :: Rabbit Daxx (CT) Antibody

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Catalog # MBS194057
Unit / Price
  0.1 mg  /  $400 +1 FREE 8GB USB
anti-DAXX antibody
Product Name

Daxx (CT), Antibody

Also Known As

Anti-Daxx (CT) Antibody

Product Synonym Names
Anti-Daxx (CT); DAXX (CT)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_000006.11 (33286335..33290793, complement). Location: 6p21.3
3D Structure
ModBase 3D Structure for Q4VX54
Host
Rabbit
Specificity
Anti-Daxx (CT) [Rabbit antibody to peptide at C-terminus of human Fas binding protein DAXX.]
This antibody recognizes full-length human, monkey, and mouse Daxx (120 kD).
Form/Format
100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Antigen
Peptide corresponding to Cterminus of human Daxx.
Stabilizer
None
Preservatives
0.02% sodium azide.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.
Other Notes
Small volumes of anti-DAXX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DAXX antibody
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain-containing receptors, TNFR1 and Fas. A novel Fas binding protein was recently cloned from human and mouse and designated Daxx. Daxx binds specifically to the Fas death domain, enhances Fas induced apoptosis, and activates the Jun Nterminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues, indicating its important function in Fas signaling pathways.
Product Categories/Family for anti-DAXX antibody
Applications Tested/Suitable for anti-DAXX antibody
Immunoblot
Application Notes for anti-DAXX antibody
Immunoblotting: use at 1:500-1:1,000 dilution.
Positive control: Whole cell lysate from HeLa cells.
NCBI/Uniprot data below describe general gene information for DAXX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,115 Da[Similar Products]
NCBI Official Full Name
Daxx
NCBI Official Synonym Full Names
death-domain associated protein
NCBI Official Symbol
NCBI Official Synonym Symbols
DAP6; EAP1; BING2; MGC126245; MGC126246
  [Similar Products]
NCBI Protein Information
death domain-associated protein 6; hDaxx; OTTHUMP00000029289; OTTHUMP00000029290; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein
UniProt Protein Name
Death-associated protein 6
UniProt Gene Name
UniProt Entry Name
Q4VX54_HUMAN
NCBI Summary for DAXX
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
UniProt Comments for DAXX
DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor; Nucleolus; Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; ubiquitin protein ligase binding; heat shock protein binding; receptor signaling protein activity; transcription corepressor activity; protein kinase activator activity

Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; viral reproduction; cytokinesis after mitosis; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; regulation of protein ubiquitination; apoptosis; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; activation of JNK activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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