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DBH elisa kit :: Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit

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Catalog # MBS703682
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Typical Testing Data/Standard Curve (for reference only)
Product Name

dopamine beta-hydroxylase (dopamine beta-monooxygenase) (DBH), ELISA Kit

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Full Product Name

Human dopamine-beta-hydroxylase, DBH ELISA Kit

Product Synonym Names
Human dopamine-beta-hydroxylase; DBH ELISA Kit; RP11-317B10.1; DBM; dopamine beta hydroxylase; dopamine beta-hydroxylase (dopamine beta-monooxygenase)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS703682 Testing Data
OMIM
609312
3D Structure
ModBase 3D Structure for P09172
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human DBH. No significant cross-reactivity or interference between human DBH and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Competitive
Detection Range
2.5 ng/ml -500 ng/ml.
Sensitivity
The minimum detectable dose of human DBH is typically less than 1.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human DBH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DBH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DBHpurchase
MBS703682 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dopamine beta-hydroxylase (dopamine beta-monooxygenase) (DBH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DBH. The ELISA analytical biochemical technique of the MBS703682 kit is based on DBH antibody-DBH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DBH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DBH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DBH elisa kit
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for DBH and Horseradish Peroxidase (HRP) conjugated DBH. The competitive inhibition reaction is launched between with HRP labeled DBH and unlabeled DBH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of DBH in the sample. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of DBH elisa kit
DBH elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for DBH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,065 Da
NCBI Official Full Name
dopamine beta-hydroxylase
NCBI Official Synonym Full Names
dopamine beta-hydroxylase (dopamine beta-monooxygenase)
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
dopamine beta-hydroxylase
UniProt Protein Name
Dopamine beta-hydroxylase
UniProt Synonym Protein Names
Dopamine beta-monooxygenaseCleaved into the following chain:Soluble dopamine beta-hydroxylase
Protein Family
UniProt Gene Name
UniProt Entry Name
DOPO_HUMAN
NCBI Summary for DBH
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]
UniProt Comments for DBH
Function: Conversion of dopamine to noradrenaline.

Catalytic activity: 3,4-dihydroxyphenethylamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O.

Cofactor: Binds 1 PQQ per subunit.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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