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DBT recombinant protein :: Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (DBT) Recombinant Protein

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Catalog # MBS948109
Unit / Price
  0.05 mg (E-Coli)  /  $835 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $1,065 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $1,120 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,235 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $1,260 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,315 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,350 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,425 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,755 +2 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $1,940 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $1,985 +2 FREE 8GB USB
  1 mg (Yeast)  /  $2,165 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $2,555 +3 FREE 8GB USB
DBT recombinant protein
Product Name

Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (DBT), Recombinant Protein

Also Known As

Recombinant Human Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (DBT)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
62-482aa, Full Length of Mature Protein
Sequence Length
482
Sequence
GQVVQFKLSD IGEGIREVTV KEWYVKEGDT VSQFDSICEV QSDKASVTIT SRYDGVIKKL YYNLDDIAYV GKPLVDIETE ALKDSEEDVV ETPAVSHDEH THQEIKGRKT LATPAVRRLA MENNIKLSEV VGSGKDGRIL KEDILNYLEK QTGAILPPSP KVEIMPPPPK PKDMTVPILV SKPPVFTGKD KTEPIKGFQK AMVKTMSAAL KIPHFGYCDE IDLTELVKLR EELKPIAFAR GIKLSFMPFF LKAASLGLLQ FPILNASVDE NCQNITYKAS HNIGIAMDTE QGLIVPNVKN VQICSIFDIA TELNRLQKLG SVGQLSTTDL TGGTFTLSNI GSIGGTFAKP VIMPPEVAIG ALGSIKAIPR FNQKGEVYKA QIMNVSWSAD HRVIDGATMS RFSNLWKSYL ENPAFMLLDL K
OMIM
248600
3D Structure
ModBase 3D Structure for P11182
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Human
Storage Buffer
Tris-based buffer, 50% glycerol
Tag Information
Tag type will be determined during the manufacturing process
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of DBT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DBT recombinant protein
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
NCBI/Uniprot data below describe general gene information for DBT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,487 Da
NCBI Official Full Name
lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
NCBI Official Synonym Full Names
dihydrolipoamide branched chain transacylase E2
NCBI Official Symbol
NCBI Official Synonym Symbols
E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCOADC-E2
  [Similar Products]
NCBI Protein Information
lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
UniProt Protein Name
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
UniProt Synonym Protein Names
52 kDa mitochondrial autoantigen of primary biliary cirrhosis
UniProt Gene Name
UniProt Synonym Gene Names
BCATE2; BCKAD-E2; BCKADE2  [Similar Products]
NCBI Summary for DBT
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for DBT
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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