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DCD elisa kit :: Human dermcidin ELISA Kit

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Catalog # MBS700974
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Typical Testing Data/Standard Curve (for reference only)
Product Name

dermcidin (DCD), ELISA Kit

Popular Item
Full Product Name

Human Proteolysis Inducing Factor/Dermcidin, PIF/DCD ELISA Kit

Product Synonym Names
Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit; AIDD; DCD-1; DSEP; HCAP; MGC71930; PIF; diffusible survival/evasion peptide; preproteolysin; proteolysis inducing factor; survival promoting peptide; dermcidin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS700974 Testing Data
OMIM
606634
3D Structure
ModBase 3D Structure for P81605
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PIF/DCD. No significant cross-reactivity or interference between human PIF/DCD and analogues was observed.
Samples
Serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range
0.625 ng/ml -40 ng/mL
Sensitivity
<0.156 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DCD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DCDpurchase
MBS700974 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dermcidin (DCD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DCD. The ELISA analytical biochemical technique of the MBS700974 kit is based on DCD antibody-DCD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DCD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DCD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DCD elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIF/DCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIF/DCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIF/DCD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIF/DCD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of DCD elisa kit
DCD elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700974. Click to request current manual
NCBI/Uniprot data below describe general gene information for DCD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,284 Da
NCBI Official Full Name
dermcidin isoform 1 preproprotein
NCBI Official Synonym Full Names
dermcidin
NCBI Official Symbol
NCBI Official Synonym Symbols
PIF; AIDD; DSEP; HCAP; DCD-1
  [Similar Products]
NCBI Protein Information
dermcidin; preproteolysin; survival promoting peptide; proteolysis inducing factor; diffusible survival/evasion peptide
UniProt Protein Name
Dermcidin
UniProt Synonym Protein Names
PreproteolysinCleaved into the following 2 chains:Survival-promoting peptide; DCD-1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AIDD; DSEP  [Similar Products]
UniProt Entry Name
DCD_HUMAN
NCBI Summary for DCD
This gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
UniProt Comments for DCD
Function: DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity.1 PublicationManual assertion based on experiment in:Ref.5

Catalytic activity: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor: Manganese. Required for survival-promoting peptide.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with DCD elisa kit
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