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DCN clia kit :: Rat DCN (Decorin) CLIA Kit

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Catalog # MBS2532227
Unit / Price
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  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

DCN (Decorin), CLIA Kit

Full Product Name

Rat DCN (Decorin) CLIA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
125255
Species Reactivity
Specificity
This kit recognizes natural and recombinant DCN. No significant cross-reactivity or interference between DCN and analogues was observed.
Assay Type
Sandwich
Detection Range
3.125-200pg/mL
Sensitivity
Min: 1.875pg/mL; Max: 200pg/mL
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DCN clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DCN clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of DCN concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to DCN. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DCN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of DCN. You can calculate the concentration of DCN in the samples by comparing the RLU value of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of DCN clia kit
DCN clia kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2532227. Click to request current manual
NCBI/Uniprot data below describe general gene information for DCN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
8,266 Da
NCBI Official Full Name
decorin
NCBI Official Synonym Full Names
decorin
NCBI Official Symbol
NCBI Official Synonym Symbols
CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
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NCBI Protein Information
decorin
UniProt Protein Name
Decorin
UniProt Synonym Protein Names
Bone proteoglycan II; PG-S2; PG40
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SLRR1B  [Similar Products]
UniProt Entry Name
PGS2_HUMAN
NCBI Summary for DCN
The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for DCN
DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; Golgi lumen; extracellular region; collagen type VI

Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: extracellular matrix organization and biogenesis; skeletal muscle development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; wound healing; response to lipopolysaccharide; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; carbohydrate metabolic process; chondroitin sulfate catabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; positive regulation of autophagy; placenta development; aging

Disease: Corneal Dystrophy, Congenital Stromal
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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