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DCPS sirna

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Catalog # MBS8206733
Unit / Price
  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
DCPS sirna
Product Name

DCPS, siRNA

Also Known As

DCPS siRNA (Rat)

Product Synonym Names
DCS1; HINT5; m7GpppX diphosphatase; DCS-1; Decapping scavenger enzyme; Hint-related 7meGMP-directed hydrolase; Histidine triad nucleotide-binding protein 5; Histidine triad protein member 5; HINT-5; Scavenger mRNA-decapping enzyme DcpS
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
336
3D Structure
ModBase 3D Structure for Q8K4F7
Host
Synthetic
Species Reactivity
Rat
Specificity
DCPS siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DCPS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DCPS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DCPS sirna
siRNA to inhibit DCPS expression using RNA interference
Applications Tested/Suitable for DCPS sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DCPS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
38,714 Da
NCBI Official Full Name
m7GpppX diphosphatase
NCBI Official Synonym Full Names
decapping enzyme, scavenger
NCBI Official Symbol
NCBI Official Synonym Symbols
Hint-5
  [Similar Products]
NCBI Protein Information
m7GpppX diphosphatase
UniProt Protein Name
m7GpppX diphosphatase
UniProt Synonym Protein Names
DCS-1; Decapping scavenger enzyme; Hint-related 7meGMP-directed hydrolase; Histidine triad nucleotide-binding protein 5; Histidine triad protein member 5; HINT-5; Scavenger mRNA-decapping enzyme DcpS
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Dcs1; Hint5; HINT-5  [Similar Products]
UniProt Entry Name
DCPS_RAT
NCBI Summary for DCPS
human homolog is a scavenger pyrophosphatase that hydrolyzes the residual cap structure following 3 to 5 decay of an mRNA [RGD, Feb 2006]
UniProt Comments for DCPS
DCPS: Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides. Belongs to the HIT family.

Protein type: Hydrolase; RNA splicing; EC 3.6.1.59

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: m7G(5')pppN diphosphatase activity; RNA 7-methylguanosine cap binding

Biological Process: deadenylation-dependent decapping; negative regulation of programmed cell death; nuclear mRNA cis splicing, via U2-type spliceosome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withDCPS sirna
 Reference Product  PubMed Publications
 Xrn1 sirna  >4 publications with DCPS and Xrn1
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