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DCTD recombinant protein :: dCMP Deaminase Recombinant Protein

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Catalog # MBS143970
Unit / Price
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  0.005 mg  /  $140 +1 FREE 8GB USB
  0.025 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $2,235 +2 FREE 8GB USB
DCTD recombinant protein
Product Name

dCMP Deaminase (DCTD), Recombinant Protein

Full Product Name

Recombinant Human dCMP Deaminase

Product Synonym Names
DCTD Human; dCMP Deaminase Human Recombinant; Deoxycytidylate deaminase; EC 3.5.4.12; dCMP Deaminase; DCTD; MGC111062
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHH HHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ
OMIM
607638
3D Structure
ModBase 3D Structure for P32321
Host
E Coli
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
The DCTD solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA & 20% glycerol.
Sterile filtered colorless solution.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Other Notes
Small volumes of DCTD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DCTD recombinant protein
Description: DCTD Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-178 a.a.) and having a molecular mass of 22.1 kDa. The DCTD is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: DCTD is an allosteric enzyme that exists as a homohexamer and is part of the cytidine and deoxycytidylate deaminase protein family. DTCD uses zinc as a cofactor to catalyze the deamination of dCMP to dUMP, thus making the nucleotide substrate (dUMP) that is used by thymidylate synthase.
NCBI/Uniprot data below describe general gene information for DCTD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,014 Da
NCBI Official Full Name
deoxycytidylate deaminase isoform a
NCBI Official Synonym Full Names
dCMP deaminase
NCBI Official Symbol
NCBI Protein Information
deoxycytidylate deaminase
UniProt Protein Name
Deoxycytidylate deaminase
UniProt Synonym Protein Names
dCMP deaminase
Protein Family
UniProt Gene Name
UniProt Entry Name
DCTD_HUMAN
NCBI Summary for DCTD
The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for DCTD
DCTD: catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.

Protein type: Nucleotide Metabolism - pyrimidine; EC 3.5.4.12; Hydrolase

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cytosol

Molecular Function: zinc ion binding; dCMP deaminase activity

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; nucleotide biosynthetic process; pyrimidine nucleoside biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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