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DDIT3 elisa kit :: Bovine DNA Damage Inducible Transcript 3 ELISA Kit

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Catalog # MBS746415
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

DNA Damage Inducible Transcript 3 (DDIT3), ELISA Kit

Full Product Name

Bovine DNA Damage Inducible Transcript 3 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613488
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Intended Uses
This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DDIT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications
!Intended Uses
This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DDIT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DDIT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDDIT3purchase
MBS746415 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA Damage Inducible Transcript 3 (DDIT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDIT3. The ELISA analytical biochemical technique of the MBS746415 kit is based on DDIT3 antibody-DDIT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDIT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDIT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DDIT3 elisa kit
Principle of the Assay: DDIT3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DDIT3. Standards or samples are then added to the microtiter plate wells and DDIT3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DDIT3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DDIT3 are added to each well to "sandwich" the DDIT3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DDIT3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDIT3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for DDIT3 elisa kit

Typical Testing Data/Standard Curve (for reference only) of DDIT3 elisa kit
DDIT3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS746415. Click to request current manual
NCBI/Uniprot data below describe general gene information for DDIT3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,700 Da
NCBI Official Full Name
DNA damage-inducible transcript 3 protein isoform 2
NCBI Official Synonym Full Names
DNA-damage-inducible transcript 3
NCBI Official Symbol
DDIT3  [Similar Products]
NCBI Official Synonym Symbols
CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
  [Similar Products]
NCBI Protein Information
DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153
UniProt Protein Name
DNA damage-inducible transcript 3 protein
UniProt Synonym Protein Names
C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153
UniProt Gene Name
DDIT3  [Similar Products]
UniProt Synonym Gene Names
CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10  [Similar Products]
UniProt Entry Name
DDIT3_HUMAN
NCBI Summary for DDIT3
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
UniProt Comments for DDIT3
CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Autophagy

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; cytosol; nucleus

Molecular Function: protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription in response to stress; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; positive regulation of interleukin-8 production; response to starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; cell redox homeostasis; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; cell cycle arrest; inhibition of CREB transcription factor; response to DNA damage stimulus; ER overload response

Disease: Myxoid Liposarcoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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