• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DDIT4 elisa kit :: Mouse DNA Damage-Inducible Transcript 4 (DDIT4) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS9354284
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
DDIT4 elisa kit
Product Name

DNA Damage-Inducible Transcript 4 (DDIT4), ELISA Kit

Popular Item
Full Product Name

Mouse DNA Damage-Inducible Transcript 4 (DDIT4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
607729
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Samples
undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples
Assay Type
Quantitative Sandwich
Detection Range
0.625 ng/ml - 20 ng/mL
Sensitivity
0.1 ng/mL
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DDIT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDDIT4purchase
MBS9354284 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA Damage-Inducible Transcript 4 (DDIT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDIT4. The ELISA analytical biochemical technique of the MBS9354284 kit is based on DDIT4 antibody-DDIT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDIT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDIT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DDIT4 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of DDIT4 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9354284. Click to request current manual
NCBI/Uniprot data below describe general gene information for DDIT4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,371 Da
NCBI Official Full Name
DNA damage-inducible transcript 4 protein
NCBI Official Synonym Full Names
DNA damage inducible transcript 4
NCBI Official Symbol
DDIT4  [Similar Products]
NCBI Official Synonym Symbols
Dig2; REDD1; REDD-1
  [Similar Products]
NCBI Protein Information
DNA damage-inducible transcript 4 protein
UniProt Protein Name
DNA damage-inducible transcript 4 protein
UniProt Synonym Protein Names
HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1
UniProt Gene Name
DDIT4  [Similar Products]
UniProt Synonym Gene Names
REDD1; RTP801; REDD-1  [Similar Products]
UniProt Entry Name
DDIT4_HUMAN
UniProt Comments for DDIT4
DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: cytoplasm; cytosol; intracellular; mitochondrion

Biological Process: brain development; cell proliferation; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; gene expression; negative regulation of glycolysis; negative regulation of peptidyl-serine phosphorylation; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; neuron differentiation; neuron migration; protein complex disassembly; response to hypoxia; transcription initiation from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000