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DDR2 recombinant protein

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Catalog # MBS637031
Unit / Price
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  0.05 mg  /  $710 +1 FREE 8GB USB
DDR2 recombinant protein
Product Name

DDR2, Recombinant Protein

Full Product Name

DDR2, Recombinant, Human (Discoidin Domain Receptor 2)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (162602228..162756409). Location: 1q23.3
OMIM
191311
3D Structure
ModBase 3D Structure for Q16832
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS.
Activity
Measured by its ability to bind collagen I (1ug/ml, 100 L/well) in a functional ELISA with an apparent Kd < 10 nM.
Endotoxin Level
< 1.0 EU per 1 g of the protein as determined by the LAL method.
Reconstitution
It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Preparation and Storage
Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of DDR2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DDR2 recombinant protein
DDR-2, also known as TYR010 and TKT, is a widely expressed 130kD type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR-2 consists of a 378 aa extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR-2 shares 53% aa sequence identity with DDR-1 and 97% aa sequence identity with mouse DDR-2. The discoidin-like domain mediates DDR-2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR-2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR-2 binding, and the D1 period is additionally required to trigger DDR-2 autophosphorylation (6). The ECD of DDR-2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR-2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR-2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR-2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR-2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR-2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR-2 itself (3, 8, 10). DDR-2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR-2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).
NCBI/Uniprot data below describe general gene information for DDR2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Based on N-terminal amino acid sequencing, the sequence was blocked, suggesting that the recombinant human DDR-2 may start at Gln 24. The calculated molecular mass of the mature recombinant protein is approximately 43.3 kD. As a result of glycosylation, t[Similar Products]
NCBI Official Full Name
discoidin domain-containing receptor 2
NCBI Official Synonym Full Names
discoidin domain receptor tyrosine kinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
TKT; MIG20a; NTRKR3; TYRO10
  [Similar Products]
NCBI Protein Information
discoidin domain-containing receptor 2; tyrosylprotein kinase; hydroxyaryl-protein kinase; discoidin domain receptor 2; tyrosine-protein kinase TYRO10; CD167 antigen-like family member B; cell migration-inducing protein 20; migration-inducing gene 16 protein; receptor protein-tyrosine kinase TKT; discoidin domain receptor family, member 2; neurotrophic tyrosine kinase receptor related 3; neurotrophic tyrosine kinase, receptor-related 3; discoidin domain-containing receptor tyrosine kinase 2
UniProt Protein Name
Discoidin domain-containing receptor 2
UniProt Synonym Protein Names
CD167 antigen-like family member B; Discoidin domain-containing receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10
UniProt Gene Name
UniProt Synonym Gene Names
NTRKR3; TKT; TYRO10; Discoidin domain receptor 2  [Similar Products]
UniProt Entry Name
DDR2_HUMAN
NCBI Summary for DDR2
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
UniProt Comments for DDR2
DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; DDR family

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: collagen binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: positive regulation of osteoblast differentiation; extracellular matrix organization and biogenesis; ossification; positive regulation of fibroblast proliferation; peptidyl-tyrosine phosphorylation; collagen fibril organization; biomineral formation; protein amino acid autophosphorylation; positive regulation of protein kinase activity; positive regulation of transcription factor activity; cell adhesion; signal transduction; regulation of bone mineralization

Disease: Spondylometaepiphyseal Dysplasia, Short Limb-hand Type
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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