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DDX11 sirna

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Catalog # MBS8209012
Unit / Price
  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
DDX11 sirna
Product Name

DDX11, siRNA

Also Known As

DDX11 siRNA (Human)

Product Synonym Names
CHL1; CHLR1; KRG2; Probable ATP-dependent RNA helicase DDX11; CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
970
OMIM
601150
3D Structure
ModBase 3D Structure for Q96FC9
Host
Synthetic
Species Reactivity
Human
Specificity
DDX11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DDX11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DDX11 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DDX11 sirna
siRNA to inhibit DDX11 expression using RNA interference
Applications Tested/Suitable for DDX11 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DDX11. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32,951 Da
NCBI Official Full Name
probable ATP-dependent RNA helicase DDX11 isoform 3
NCBI Official Synonym Full Names
DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
NCBI Official Symbol
DDX11  [Similar Products]
NCBI Official Synonym Symbols
CHL1; KRG2; WABS; CHLR1
  [Similar Products]
NCBI Protein Information
probable ATP-dependent RNA helicase DDX11
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX11
UniProt Synonym Protein Names
CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2
UniProt Gene Name
DDX11  [Similar Products]
UniProt Synonym Gene Names
CHL1; CHLR1; KRG2; hCHLR1; KRG-2  [Similar Products]
UniProt Entry Name
DDX11_HUMAN
NCBI Summary for DDX11
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for DDX11
DDX11: DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS). It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist. Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Nucleolus; Helicase

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; spindle pole; nuclear chromatin; cytoplasm; nucleolus; midbody; nucleus

Molecular Function: ATP-dependent DNA helicase activity; DNA-dependent ATPase activity; protein binding; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; double-stranded DNA binding; helicase activity; single-stranded DNA binding; ATP binding

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; viral reproduction; unfolded protein response; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; sister chromatid cohesion; DNA duplex unwinding

Disease: Warsaw Breakage Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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