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DEFA1B recombinant protein :: DEFENSIN ALPHA 1 Recombinant Protein

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Catalog # MBS232451
Unit / Price
  0.02 mg  /  $450 +1 FREE 8GB USB
DEFA1B recombinant protein
Product Name

DEFENSIN ALPHA 1 (DEFA1B), Recombinant Protein

Also Known As

RECOMBINANT HUMAN DEFENSIN ALPHA 1

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
94
OMIM
125220
3D Structure
ModBase 3D Structure for P59665
Purity/Purification
>98% by SDS PAGE and HPLC analysis
Form/Format
Purified recombinant protein - lyophilised
Concentration
Approximate Protein Concentration: 0.1mg/ml after reconstitution (lot specific)
Reconstitution
Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Preparation
Activity
Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0-10.0 ng/ml.
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Other Notes
Small volumes of DEFA1B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DEFA1B recombinant protein
Recombinant Human Defensin-alpha-1 is a 3.4kDa peptide comprised of 30 amino acid residues corresponding to full-length mature human defenstin alpha 1. Defensin-alpha-1, otherwise known as HNP-1, is a secreted cationic antimicrobial peptide and member of the alpha-defensin family, which plays an important role in the innate immune response against microbial infections.
Applications Tested/Suitable for DEFA1B recombinant protein
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes for DEFA1B recombinant protein
ELISA: Recombinant human defensin-alpha-1 may be used as a standard for ELISA applications with either a purified anti human defensin-alpha-1 antibody or a biotinylated anti human defensin-alpha-1 antibody.
Western Blot: Recombinant human defensin-alpha-1 may be used as a positive control for Western Blotting applications with either a purifed human defensin-alpha-1 antibody or a biotinylated human defensin-alpha-1 antibody.
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
NCBI/Uniprot data below describe general gene information for DEFA1B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
3.4kD (30 amino acid residues)[Similar Products]
NCBI Official Full Name
neutrophil defensin 1 isoform 2 preproprotein
NCBI Official Synonym Full Names
defensin, alpha 1B
NCBI Official Symbol
DEFA1B  [Similar Products]
NCBI Official Synonym Symbols
HP1; HP-1; HNP-1
  [Similar Products]
NCBI Protein Information
neutrophil defensin 1
UniProt Protein Name
Neutrophil defensin 1
UniProt Synonym Protein Names
Defensin, alpha 1; HNP-1; HP-1; HP1
UniProt Gene Name
DEFA1  [Similar Products]
UniProt Synonym Gene Names
DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2  [Similar Products]
UniProt Entry Name
DEF1_HUMAN
NCBI Summary for DEFA1B
Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
UniProt Comments for DEFA1B
defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to virus; defense response to fungus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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