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DEFB103B elisa kit :: Human Defensin Beta 103B (DEFB103B) ELISA Kit

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Catalog # MBS2020601 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Defensin Beta 103B (DEFB103B), ELISA Kit

Popular Item
Full Product Name

Defensin Beta 103B (DEFB103B) ELISA Kit

Product Synonym Names
HBP3; HBD3; BD3; DEFB103; DEFB3; Defensin-like protein; Beta-defensin 3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
606611
3D Structure
ModBase 3D Structure for P81534
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Defensin Beta 103B (DEFB103B).
No significant cross-reactivity or interference between Defensin Beta 103B (DEFB103B) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
31.25-2000pg/mL
Sensitivity
< 12.7pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Defensin Beta 103B (DEFB103B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Defensin Beta 103B (DEFB103B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DEFB103B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDEFB103Bpurchase
MBS2020601 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin Beta 103B (DEFB103B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DEFB103B. The ELISA analytical biochemical technique of the MBS2020601 kit is based on DEFB103B antibody-DEFB103B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFB103B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFB103B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DEFB103B elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103B (DEFB103B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103B (DEFB103B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103B (DEFB103B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 103B (DEFB103B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for DEFB103B elisa kit

Typical Testing Data/Standard Curve (for reference only) of DEFB103B elisa kit
DEFB103B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020601. Click to request current manual
NCBI/Uniprot data below describe general gene information for DEFB103B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
7,697 Da
NCBI Official Full Name
Defensin, beta 103B, partial
NCBI Official Synonym Full Names
defensin, beta 103A
NCBI Official Symbol
DEFB103A  [Similar Products]
NCBI Official Synonym Symbols
BD-3; HBD3; HBP3; DEFB3; HBP-3; hBD-3; DEFB-3; DEFB103
  [Similar Products]
NCBI Protein Information
beta-defensin 103
UniProt Protein Name
Beta-defensin 103
UniProt Synonym Protein Names
Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein
UniProt Gene Name
DEFB103A  [Similar Products]
UniProt Synonym Gene Names
BD3; DEFB103; DEFB3; BD-3; DEFB-3; HBD3; hBD-3  [Similar Products]
UniProt Entry Name
D103A_HUMAN
NCBI Summary for DEFB103B
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans. [provided by RefSeq, Oct 2014]
UniProt Comments for DEFB103B
defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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