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DEFbeta1 elisa kit :: Human Beta-Defensins 1 (DEFbeta1) ELISA Kit

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Catalog # MBS2601339
Unit / Price
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  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Beta-Defensins 1 (DEFbeta1), ELISA Kit

Popular Item
Full Product Name

Human Beta-Defensins 1 (DEFbeta1) ELISA Kit

Product Synonym Names
Human Beta-Defensins 1 (DEFb1) ELISA Kit
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602056
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant DEFbeta1 concentration.
Assay Type
Quantitative Sandwich
Sensitivity
Up to 12 pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DEFbeta1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DEFbeta1purchase
MBS2601339 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beta-Defensins 1 (DEFbeta1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DEFbeta1. The ELISA analytical biochemical technique of the MBS2601339 kit is based on DEFbeta1 antibody-DEFbeta1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFbeta1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFbeta1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DEFbeta1 elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human DEFbeta1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve of DEFbeta1 elisa kit
DEFbeta1 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS2601339. Click to request current manual
NCBI/Uniprot data below describe general gene information for DEFbeta1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
7,420 Da
NCBI Official Full Name
Beta-defensin 1
NCBI Official Synonym Full Names
defensin, beta 1
NCBI Official Symbol
DEFB1  [Similar Products]
NCBI Official Synonym Symbols
BD1; HBD1; DEFB-1; DEFB101
  [Similar Products]
NCBI Protein Information
beta-defensin 1; BD-1; beta-defensin-1
UniProt Protein Name
Beta-defensin 1
UniProt Synonym Protein Names
Defensin, beta 1
UniProt Gene Name
DEFB1  [Similar Products]
UniProt Synonym Gene Names
BD1; HBD1; BD-1; hBD-1  [Similar Products]
UniProt Entry Name
DEFB1_HUMAN
NCBI Summary for DEFbeta1
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]
UniProt Comments for DEFbeta1
defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: G-protein coupled receptor protein signaling pathway; defense response to Gram-positive bacterium; innate immune response in mucosa; response to bacterium; antibacterial humoral response; innate immune response; immune response; response to testosterone stimulus; chemotaxis; acute inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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