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DENND3 sirna

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Catalog # MBS8214485
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
DENND3 sirna
Product Name

DENND3, siRNA

Full Product Name

DENND3 siRNA (Mouse)

Product Synonym Names
KIAA0870; DENN domain-containing protein 3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for A2RT67
Host
Synthetic
Species Reactivity
Mouse
Specificity
DENND3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DENND3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DENND3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DENND3 sirna
siRNA to inhibit DENND3 expression using RNA interference
Applications Tested/Suitable for DENND3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DENND3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
143,888 Da
NCBI Official Full Name
DENN domain-containing protein 3
NCBI Official Synonym Full Names
DENN/MADD domain containing 3
NCBI Official Symbol
Dennd3  [Similar Products]
NCBI Official Synonym Symbols
AI447457; E030003N15Rik
  [Similar Products]
NCBI Protein Information
DENN domain-containing protein 3
UniProt Protein Name
DENN domain-containing protein 3
UniProt Gene Name
Dennd3  [Similar Products]
UniProt Synonym Gene Names
Kiaa0870  [Similar Products]
UniProt Entry Name
DEND3_MOUSE
UniProt Comments for DENND3
DENND3: a guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Plays a role in protein transport from recycling endosomes to lysosomes regulating, for example, the degradation of the transferrin receptor. Four isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rab; GEFs

Cellular Component: cell; intracellular

Molecular Function: guanyl-nucleotide exchange factor activity; Rab guanyl-nucleotide exchange factor activity

Biological Process: cellular protein catabolic process; endosome to lysosome transport; positive regulation of GTPase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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