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DHCR7 elisa kit :: Human 7-dehydrocholesterol reductase ELISA Kit

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Catalog # MBS945050
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

7-dehydrocholesterol reductase (DHCR7), ELISA Kit

Popular Item
Full Product Name

Human 7-dehydrocholesterol reductase, DHCR7 ELISA Kit

Product Synonym Names
Human 7-dehydrocholesterol reductase (DHCR7) ELISA kit; SLOS; delta-7-dehydrocholesterol reductase; sterol delta-7-reductase; 7-dehydrocholesterol reductase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS945050 Testing Data
OMIM
602858
3D Structure
ModBase 3D Structure for Q9UBM7
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human DHCR7. No significant cross-reactivity or interference between human DHCR7 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml.
Sensitivity
The minimum detectable dose of human DHCR7 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DHCR7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DHCR7purchase
MBS945050 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 7-dehydrocholesterol reductase (DHCR7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHCR7. The ELISA analytical biochemical technique of the MBS945050 kit is based on DHCR7 antibody-DHCR7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHCR7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHCR7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DHCR7 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DHCR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHCR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of DHCR7 elisa kit
DHCR7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS945050. Click to request current manual
NCBI/Uniprot data below describe general gene information for DHCR7. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
54,489 Da
NCBI Official Full Name
7-dehydrocholesterol reductase
NCBI Official Synonym Full Names
7-dehydrocholesterol reductase
NCBI Official Symbol
DHCR7  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
7-dehydrocholesterol reductase; 7-DHC reductase; sterol delta-7-reductase; putative sterol reductase SR-2; delta-7-dehydrocholesterol reductase
UniProt Protein Name
7-dehydrocholesterol reductase
UniProt Synonym Protein Names
Putative sterol reductase SR-2; Sterol Delta(7)-reductase
UniProt Gene Name
DHCR7  [Similar Products]
UniProt Synonym Gene Names
D7SR; 7-DHC reductase  [Similar Products]
UniProt Entry Name
DHCR7_HUMAN
NCBI Summary for DHCR7
This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]
UniProt Comments for DHCR7
DHCR7: Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC). Defects in DHCR7 are the cause of Smith-Lemli-Opitz syndrome (SLOS); also known as SLO syndrome or RSH syndrome. SLOS is an autosomal recessive frequent inborn disorder of sterol metabolism with characteristic congenital malformations and dysmorphias. All patients suffer from mental retardation. Children with SLOS have elevated serum 7-dehydrocholesterol (7- DHC) levels and low serum cholesterol levels. SLOS occurs in relatively high frequency: approximately 1 in 20,000 to 30,000 births in populations of northern and central European background. Historically, a clinical distinction often was made between classic ('type I') SLOS and the more severely affected ('type II') patients. There is, in reality, a clinical and biochemical continuum from mild to severe SLOS. Belongs to the ERG4/ERG24 family.

Protein type: Membrane protein, multi-pass; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; EC 1.3.1.21; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: 7-dehydrocholesterol reductase activity

Biological Process: blood vessel development; multicellular organism growth; regulation of cholesterol biosynthetic process; cell differentiation; cholesterol biosynthetic process; regulation of cell proliferation; post-embryonic development; lung development

Disease: Smith-lemli-opitz Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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