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DHEA elisa kit :: Human Dehydroepiandrosterone (DHEA) ELISA Kit

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Catalog # MBS266093
Unit / Price
  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Dehydroepiandrosterone (DHEA), ELISA Kit

Popular Item
Also Known As

Human Dehydroepiandrosterone (DHEA) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
285
OMIM
125263
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or cell culture supernatant
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/mL
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DHEA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDHEApurchase
MBS266093 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dehydroepiandrosterone (DHEA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHEA. The ELISA analytical biochemical technique of the MBS266093 kit is based on DHEA antibody-DHEA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHEA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHEA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DHEA elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human DHEA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of DHEA elisa kit
DHEA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS266093. Click to request current manual
NCBI/Uniprot data below describe general gene information for DHEA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
Molecular Weight
33,780 Da
NCBI Official Full Name
dehydroepiandrosterone sulfotransferase
NCBI Official Synonym Full Names
sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
NCBI Official Symbol
SULT2A1  [Similar Products]
NCBI Official Synonym Symbols
HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
  [Similar Products]
NCBI Protein Information
bile salt sulfotransferase; bile-salt sulfotranasferase 2A1; alcohol/hydroxysteroid sulfotransferase
UniProt Protein Name
Bile salt sulfotransferase
UniProt Synonym Protein Names
Dehydroepiandrosterone sulfotransferase; DHEA-ST; Hydroxysteroid Sulfotransferase; HST; ST2; ST2A3; Sulfotransferase 2A1; ST2A1
UniProt Gene Name
SULT2A1  [Similar Products]
UniProt Synonym Gene Names
HST; STD; DHEA-ST; HST; ST2A1  [Similar Products]
UniProt Entry Name
ST2A1_HUMAN
NCBI Summary for DHEA
This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq, Mar 2010]
UniProt Comments for DHEA
SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.14; Transferase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; protein binding; bile-salt sulfotransferase activity

Biological Process: steroid metabolic process; bile acid catabolic process; xenobiotic metabolic process; sulfation; digestion; cellular lipid metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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