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DHEA clia kit :: Mouse DHEA (Dehydroepiandrosterone) CLIA Kit

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Catalog # MBS2531205
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DHEA clia kit
Product Name

DHEA (Dehydroepiandrosterone), CLIA Kit

Full Product Name

Mouse DHEA (Dehydroepiandrosterone) CLIA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
125263
Species Reactivity
Assay Type
Competitive
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Sensitivity
0.094ng/mL
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DHEA clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DHEA clia kit
This CLIA kit uses Competitive-CLiA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse DHEA. During the reaction, Mouse DHEA in the sample or standard competes with a fixed amount of Mouse DHEA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse DHEA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is negatively associated with the concentration of Mouse DHEA. You can calculate the concentration of Mouse DHEA in the samples by comparing the RLU of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for DHEA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
Molecular Weight
33,780 Da
NCBI Official Full Name
dehydroepiandrosterone sulfotransferase
NCBI Official Synonym Full Names
sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
NCBI Official Symbol
SULT2A1  [Similar Products]
NCBI Official Synonym Symbols
HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
  [Similar Products]
NCBI Protein Information
bile salt sulfotransferase
UniProt Protein Name
Bile salt sulfotransferase
UniProt Synonym Protein Names
Dehydroepiandrosterone sulfotransferase; DHEA-ST; Hydroxysteroid Sulfotransferase; HST; ST2; ST2A3; Sulfotransferase 2A1; ST2A1
UniProt Gene Name
SULT2A1  [Similar Products]
UniProt Synonym Gene Names
HST; STD; DHEA-ST; HST; ST2A1  [Similar Products]
UniProt Entry Name
ST2A1_HUMAN
NCBI Summary for DHEA
This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq, Mar 2010]
UniProt Comments for DHEA
SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.14; Transferase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; protein binding; bile-salt sulfotransferase activity

Biological Process: steroid metabolic process; xenobiotic metabolic process; bile acid catabolic process; digestion; sulfation; cellular lipid metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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