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DHVD3 elisa kit :: General 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

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Catalog # MBS2700158 (SPECIAL PROMOTIONAL PRICING for a limited time)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

1,25-Dihydroxyvitamin D3 (DHVD3), ELISA Kit

Popular Item
Full Product Name

General 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

Product Synonym Names
DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
L13286 mRNA
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of 1,25-Dihydroxyvitamin D3 (DHVD3).
No significant cross-reactivity or interference between 1,25-Dihydroxyvitamin D3 (DHVD3) and analogues was observed.
Assay Type
Competitive Inhibition
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
123.5-10000pg/mL
Sensitivity
< 49.5pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level 1,25-Dihydroxyvitamin D3 (DHVD3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level 1,25-Dihydroxyvitamin D3 (DHVD3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DHVD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DHVD3purchase
MBS2700158 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHVD3. The ELISA analytical biochemical technique of the MBS2700158 kit is based on DHVD3 antibody-DHVD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHVD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHVD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DHVD3 elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to DHVD3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled DHVD3 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of DHVD3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of DHVD3 in the sample.

Typical Testing Data/Standard Curve (for reference only) of DHVD3 elisa kit
DHVD3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2700158. Click to request current manual
NCBI/Uniprot data below describe general gene information for DHVD3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,051 Da
NCBI Official Full Name
1,25-dihydroxyvitamin D3 24-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450 family 24 subfamily A member 1
NCBI Official Symbol
CYP24A1  [Similar Products]
NCBI Official Synonym Symbols
CP24; HCAI; CYP24; HCINF1; P450-CC24
  [Similar Products]
NCBI Protein Information
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Protein Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Synonym Protein Names
Cytochrome P450 24A1; Cytochrome P450-CC24
UniProt Gene Name
CYP24A1  [Similar Products]
UniProt Synonym Gene Names
CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase  [Similar Products]
NCBI Summary for DHVD3
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for DHVD3
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D3-26,23-lactone as end product.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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