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dinB recombinant protein :: DNA polymerase IV (dinB) Recombinant Protein

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Catalog # MBS1423494
Unit / Price
  0.05 mg (E-Coli)  /  $760 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $855 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $1,015 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $1,090 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,120 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $1,160 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,315 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,330 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,570 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,700 +2 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $2,045 +2 FREE 8GB USB
  1 mg (Yeast)  /  $2,085 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $2,155 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $3,170 +3 FREE 8GB USB
dinB recombinant protein
Product Name

DNA polymerase IV (dinB), Recombinant Protein

Popular Item
Full Product Name

Recombinant Escherichia coli DNA polymerase IV (dinB)

Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS1423494 COA
Sequence Positions
1-351aa, full length protein
Sequence
MRKIIHVDMD CFFAAVEMRD NPALRDIPIA IGGSRERRGV ISTANYPARK FGVRSAMPTG MALKLCPHLT LLPGRFDAYK EASNHIREIF SRYTSRIEPL SLDEAYLDVT DSVHCHGSAT LIAQEIRQTI FNELQLTASA GVAPVKFLAK IASDMNKPNG QFVITPAEVP AFLQTLPLAK IPGVGKVSAA KLEAMGLRTC GDVQKCDLVM LLKRFGKFGR ILWERSQGID ERDVNSERLR KSVGVERTMA EDIHHWSECE AIIERLYPEL ERRLAKVKPD LLIARQGVKL KFDDFQQTTQ EHVWPRLNKA DLIATARKTW DERRGGRGVR LVGLHVTLLD PQMERQLVLG L
3D Structure
ModBase 3D Structure for Q47155
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Escherichia coli (strain K12)
Storage Buffer
Tris-based buffer, 50% glycerol
Tag Information
Tag type will be determined during the manufacturing process
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of dinB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for dinB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
39,516 Da
NCBI Official Full Name
DNA polymerase IV
NCBI Official Symbol
NCBI Official Synonym Symbols
dinP; ECK0232; JW0221
  [Similar Products]
NCBI Protein Information
DNA polymerase IV
UniProt Protein Name
DNA polymerase IV
UniProt Synonym Protein Names
Translesion synthesis polymerase IV
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
dinP; TSL polymerase IV  [Similar Products]
NCBI Summary for dinB
Part of dinB-yafNOP operon. [More information is available at EcoGene: EG13141]. dinB encodes DNA polymerase IV (DNA pol IV), which belongs to the Y family of DNA polymerases . [More information is available at EcoCyc: G6115].
UniProt Comments for dinB
Poorly processive, error-prone DNA polymerase involved in translesion repair and untargeted mutagenesis (PubMed:10488344, PubMed:10801133). Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by Pol IV. Exhibits no 3'-5' exonuclease (proofreading) activity (PubMed:10488344). Overexpression of Pol IV results in increased frameshift mutagenesis. It is required for stationary-phase adaptive mutation, which provides the bacterium with flexibility in dealing with environmental stress, enhancing long-term survival and evolutionary fitness. Not seen to be involved in translesion snythesis even when stimulated by the beta slding-clamp and clamp-loading complex, which do however increase non-targeted DNA polymerse efficiency 3,000-fold, may be due to targeting to stalled replication forks on nondamaged DNA (PubMed:10801133, PubMed:16168375). Involved in translesional synthesis, in conjunction with the beta clamp from PolIII (PubMed:14592985, PubMed:14729336).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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