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Dio3 recombinant protein :: Type III iodothyronine deiodinase (Dio3) Recombinant Protein

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Catalog # MBS1116133
Unit / Price
  0.01 mg (E-Coli)  /  $195 +1 FREE 8GB USB
  0.01 mg (Yeast)  /  $195 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $255 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $255 +1 FREE 8GB USB
  0.1 mg (Yeast)  /  $415 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $420 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $660 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $665 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $930 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,090 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,090 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,170 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,175 +1 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $1,695 +2 FREE 8GB USB
  1 mg (E-Coli)  /  $1,725 +2 FREE 8GB USB
  1 mg (Yeast)  /  $1,730 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $1,780 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $2,260 +2 FREE 8GB USB
SDS-PAGE
Product Name

Type III iodothyronine deiodinase (Dio3), Recombinant Protein

Popular Item
Also Known As

Recombinant Rat Type III iodothyronine deiodinase (Dio3), partial

Product Synonym Names
5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
37-278aa (Missing the aa "U" at the 144 site. This is the complete extracellular domain before UniProt record was updated. Current UniProt #P49897. A cysteine mutant or serine mutant of MBS1116133 is also available upon request)
Sequence Length
278
Sequence
DFLCIRKHFL RRRHPDHPEP EVELNSEGEE MPPDDPPICV SDDNRLCTLA SLKAVWHGQK LDFFKQAHEG GPAPNSEVVR PDGFQSQRIL DYAQGTRPLV LNFGSCTPPF MARMSAFQRL VTKYQRDVDF LIIYIEEAHP SDGWVTTDSP YVIPQHRSLE DRVSAARVLQ QGAPGCALVL DTMANSSSSA YGAYFERLYV IQSGTIMYQG GRGPDGYQVS ELRTWLERYD EQLHGTRPRR L
3D Structure
ModBase 3D Structure for P49897
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of Dio3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Dio3 recombinant protein
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing prature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.

Dio3 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for Dio3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
31.5kD
NCBI Official Full Name
thyroxine 5-deiodinase
NCBI Official Synonym Full Names
deiodinase, iodothyronine, type III
NCBI Official Symbol
NCBI Official Synonym Symbols
5DIII; DIOIII
  [Similar Products]
NCBI Protein Information
thyroxine 5-deiodinase
UniProt Protein Name
Thyroxine 5-deiodinase
UniProt Synonym Protein Names
5DIII; DIOIII; Type 3 DI; Type III iodothyronine deiodinase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Itdi3; Txdi3  [Similar Products]
UniProt Entry Name
IOD3_RAT
NCBI Summary for Dio3
The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. It contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
UniProt Comments for Dio3
DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: EC 1.97.1.11; Oxidoreductase; Membrane protein, integral

Cellular Component: endosome membrane; integral to membrane; plasma membrane

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: hormone biosynthetic process; positive regulation of multicellular organism growth; response to hypoxia; thyroid hormone catabolic process; thyroid hormone metabolic process
Product References and Citations for Dio3 recombinant protein
Genome sequence of the Brown Norway rat yields insights into mammalian evolution.Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.Nature 428:493-521(2004) Cloning and expression of a cDNA for a mammalian type III iodothyronine deiodinase.Croteau W., Whittemore S.L., Schneider M.J., St Germain D.L.J. Biol. Chem. 270:16569-16575(1995)

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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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