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DLD elisa kit :: Human Dihydrolipoyl dehydrogenase, mitochondrial ELISA Kit

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Catalog # MBS2885103
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  48-Strip-Wells  /  $400 +1 FREE 8GB USB
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Typical Standard Curve/Testing Data
Product Name

Dihydrolipoyl dehydrogenase, mitochondrial (DLD), ELISA Kit

Popular Item
Full Product Name

Human Dihydrolipoyl dehydrogenase, mitochondrial ELISA Kit

Product Synonym Names
Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; DLD; GCSL; LAD; PHE3; 1.8.1.4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS2885103 Testing Data
OMIM
238331
3D Structure
ModBase 3D Structure for P09622
Species Reactivity
Assay Type
Competitive
Detection Range
0.78-50 ng/mL
Sensitivity
0.45 ng/mL
Intra-Assay CV
<=8.4%
Inter-Assay CV
<=11.1%
Recovery
91%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DLD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DLDpurchase
MBS2885103 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dihydrolipoyl dehydrogenase, mitochondrial (DLD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DLD. The ELISA analytical biochemical technique of the MBS2885103 kit is based on DLD antibody-DLD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DLD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DLD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DLD elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Standard Curve/Testing Data of DLD elisa kit
DLD elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS2885103. Click to request current manual
NCBI/Uniprot data below describe general gene information for DLD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,283 Da
NCBI Official Full Name
dihydrolipoyl dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
dihydrolipoamide dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
E3; LAD; DLDD; DLDH; GCSL; PHE3
  [Similar Products]
NCBI Protein Information
dihydrolipoyl dehydrogenase, mitochondrial
UniProt Protein Name
Dihydrolipoyl dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GCSL; LAD; PHE3  [Similar Products]
UniProt Entry Name
DLDH_HUMAN
NCBI Summary for DLD
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for DLD
DLD: a multi-functional mitochondrial enzyme. An enzymatic component of the mitochondrial glycine cleavage system, the pyruvate dehydrogenase complex (PDHC), the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Is the E3 component of the PDHC that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. The E3 component has dihydrolipoamide dehydrogenase activity. The PDHC contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. Defects in DLD are a cause of maple syrup urine disease (MSUD), characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. Differentially expressed in the Wernicke's Area from patients with schizophrenia. Inhibited by 5-methoxyindole-2-carboxylic acid (MICA).

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.8.1.4

Chromosomal Location of Human Ortholog: 7q31-q32

Cellular Component: acrosomal matrix; cilium; mitochondrial matrix; mitochondrion; myelin sheath; nucleoplasm; oxoglutarate dehydrogenase complex; pyruvate dehydrogenase complex

Molecular Function: dihydrolipoyl dehydrogenase activity; FAD binding; lipoamide binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; aging; branched chain family amino acid catabolic process; cell redox homeostasis; cellular metabolic process; dihydrolipoamide metabolic process; gastrulation; glyoxylate metabolic process; lipoate metabolic process; lysine catabolic process; mitochondrial electron transport, NADH to ubiquinone; proteolysis; pyruvate metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of membrane potential; sperm capacitation; tricarboxylic acid cycle

Disease: Dihydrolipoamide Dehydrogenase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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