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DNAJC5 elisa kit :: Mouse DnaJ (Hsp40) homolog, subfamily C, member 5 ELISA Kit

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Catalog # MBS903928
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Typical Testing Data/Standard Curve (for reference only)
Product Name

DnaJ (Hsp40) homolog, subfamily C, member 5 (DNAJC5), ELISA Kit

Popular Item
Also Known As

Mouse DnaJ homolog subfamily C member 5, DNAJC5 ELISA Kit

Product Synonym Names
Mouse DnaJ homolog subfamily C member 5 (DNAJC5) ELISA kit; CSP; DKFZp434N1429; DKFZp761N1221; DNAJC5A; FLJ00118; FLJ13070; cysteine string protein; DnaJ (Hsp40) homolog; subfamily C; member 5
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS903928 Testing Data
Sequence Length
198
3D Structure
ModBase 3D Structure for P60904
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse DNAJC5. No significant cross-reactivity or interference between Mouse DNAJC5 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
28 pg/ml-1800 pg/ml
Sensitivity
7 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DNAJC5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDNAJC5purchase
MBS903928 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DnaJ (Hsp40) homolog, subfamily C, member 5 (DNAJC5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DNAJC5. The ELISA analytical biochemical technique of the MBS903928 kit is based on DNAJC5 antibody-DNAJC5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DNAJC5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DNAJC5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DNAJC5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DNAJC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAJC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJC5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of DNAJC5 elisa kit
DNAJC5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS903928. Click to request current manual
NCBI/Uniprot data below describe general gene information for DNAJC5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,101 Da
NCBI Official Full Name
dnaJ homolog subfamily C member 5
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily C, member 5
NCBI Official Symbol
Dnajc5  [Similar Products]
NCBI Official Synonym Symbols
Csp; AU018536; 2610314I24Rik
  [Similar Products]
NCBI Protein Information
dnaJ homolog subfamily C member 5; cysteine string protein
UniProt Protein Name
DnaJ homolog subfamily C member 5
UniProt Synonym Protein Names
Cysteine string protein; CSP
Protein Family
UniProt Gene Name
Dnajc5  [Similar Products]
UniProt Synonym Gene Names
Csp; CSP  [Similar Products]
UniProt Entry Name
DNJC5_MOUSE
UniProt Comments for DNAJC5
DNAJC5: a cysteine string protein that inhibits N-type calcium channels which is blocked by huntingtin mutants. May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings. Modulates the maturation of cystic fibrosis transmembrane conductance regulator. Two splice-variant isoforms have been described.

Protein type: Vesicle

Cellular Component: synaptic vesicle; mitochondrion; membrane; lysosomal membrane; plasma membrane; terminal button

Molecular Function: ATP-dependent protein binding; glycoprotein binding

Biological Process: negative regulation of neuron apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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