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DNASE2 enzyme :: Deoxyribonuclease II, Porcine spleen Enzyme

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Catalog # MBS653670
Unit / Price
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  20000 Units  /  $560 +1 FREE 8GB USB
DNASE2 enzyme
Product Name

Deoxyribonuclease II, Porcine spleen (DNASE2), Enzyme

Popular Item
Full Product Name

Deoxyribonuclease II, Porcine spleen (DNase II)

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_010444.3 (66447514..66450301, complement). Location: chromosome: 2
3D Structure
ModBase 3D Structure for O62855
Host
Porcine pancreas
Purity/Purification
Molecular Biology Grade
Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966).
Form/Format
Supplied as a chromatographically purified, dialyzed, lyophilized powder.
CAS Number
9003-98-9
Activity
U/mg: 39,912
dw: u/mgP: 46,409
Country of Origin
US certified starting material, collected in USDA approved facilities
% Protein
86%
Unit Definition
1 unit causes an increase in absorbance at 260nm of 0.001 per minute per minute at 25 degree C, pH4.6 using highly polymerized DNA as substate.
Quality Control
SDS-PAGE
Unit Definition
1 unit causes an increase in absorbance at 260 nm of 0.002 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 4.6.
Note: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37 degree C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 6 months after receipt at -20 degree C. For maximum recofery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of DNASE2 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DNASE2 enzyme
Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
Product Categories/Family for DNASE2 enzyme
Application Notes for DNASE2 enzyme
DNA Sequencing (acidic pH), DNA Digestion (acidic pH)
NCBI/Uniprot data below describe general gene information for DNASE2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
Deoxyribonuclease II
NCBI Official Symbol
DNASE2  [Similar Products]
NCBI Protein Information
deoxyribonuclease-2-alpha; acid DNase; DNase II alpha; lysosomal DNase II; deoxyribonuclease II alpha
UniProt Protein Name
Deoxyribonuclease-2-alpha
UniProt Synonym Protein Names
Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II
Protein Family
UniProt Gene Name
DNASE2  [Similar Products]
UniProt Synonym Gene Names
DNASE2A; DNL2; DNase II alpha  [Similar Products]
UniProt Entry Name
DNS2A_PIG
UniProt Comments for DNASE2
Function: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells

By similarity.

Catalytic activity: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Subcellular location: Lysosome.

Miscellaneous: Not required for the generation of the characteristic DNA fragmentation observed in apoptotic cells, but for the degradation of DNA from dying cells.

Sequence similarities: Belongs to the DNase II family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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