Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List DOC elisa kit :: Human Deoxycorticosterone ELISA Kit Catalog # MBS749262 Unit / Price 48-Strip-Wells / $440 +1 FREE 8GB USB 96-Strip-Wells / $640 +1 FREE 8GB USB 5x96-Strip-Wells / $2,895 +3 FREE 8GB USB 10x96-Strip-Wells / $5,415 +6 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Deoxycorticosterone (DOC), ELISA Kit Popular Item Full Product Name Human Deoxycorticosterone ELISA Kit Product Gene Name DOC elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Request for Current Manual Insert Request Current Manual Species Reactivity Human Specificity This assay has high sensitivity and excellent specificity for detection of DOC. No significant cross-reactivity or interference between DOC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DOC and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate Assay Type Competitive Detection Range 250-5000pg/mL Sensitivity 1.0pg/mL Intended Uses This DOC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DOC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team Product Note Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual. Other Notes Small volumes of DOC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms forDOCpurchase MBS749262 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Deoxycorticosterone (DOC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DOC. The ELISA analytical biochemical technique of the MBS749262 kit is based on DOC antibody-DOC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DOC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DOC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for DOC elisa kit Principle of the assay: DOC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DOC antibody and an DOC-HRP conjugate. The assay sample and buffer are incubated together with DOC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DOC concentration since DOC from samples and DOC-HRP conjugate compete for the anti-DOC antibody binding site. Since the number of sites is limited, as more sites are occupied by DOC from the sample, fewer sites are left to bind DOC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DOC concentration in each sample is interpolated from this standard curve. Typical Testing Data/Standard Curve (for reference only) of DOC elisa kit TOP Sample Manual Insert of MBS749262. Click to request current manual Protein Family Toxin Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP
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