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DPD elisa kit :: Mouse DPD ELISA Kit

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Catalog # MBS2503693
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

DPD, ELISA Kit

Popular Item
Also Known As

Mouse DPD (Deoxypyridinoline) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2503693 COA
Sequence Length
1025
3D Structure
ModBase 3D Structure for O89000
Species Reactivity
Specificity
This kit recognizes natural and some recombinant Mouse DPD. No significant crossreactivity or interference between Mouse DPD and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
3.125-200ng/mL
Sensitivity
Min: 1.875ng/mL; Max: 200ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DPD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDPDpurchase
MBS2503693 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DPD, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPD. The ELISA analytical biochemical technique of the MBS2503693 kit is based on DPD antibody-DPD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DPD elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse DPD concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DPD. During the reaction, Mouse DPD in the sample or standard competes with a fixed amount of Mouse DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the Substrate Reagent is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change can be measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse DPD in samples can be calculated by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of DPD elisa kit
DPD elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2503693. Click to request current manual
NCBI/Uniprot data below describe general gene information for DPD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
111,468 Da
NCBI Official Full Name
dihydropyrimidine dehydrogenase
NCBI Official Synonym Full Names
dihydropyrimidine dehydrogenase
NCBI Official Symbol
NCBI Protein Information
dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase
UniProt Protein Name
Dihydropyrimidine dehydrogenase [NADP(+)]
UniProt Synonym Protein Names
Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase
UniProt Gene Name
UniProt Synonym Gene Names
DPD; DHPDHase; DPD  [Similar Products]
UniProt Entry Name
DPYD_RAT
NCBI Summary for DPD
catalyzes the conversion of 5,6-dihydrouracil and NADP+ to uracil, NADPH, and H+ in pyrimidine degradation [RGD, Feb 2006]
UniProt Comments for DPD
DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine; EC 1.3.1.2; Oxidoreductase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Xenobiotic Metabolism - drug metabolism - other enzymes

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; uracil binding; dihydroorotate dehydrogenase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; iron-sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: response to drug; uracil metabolic process; circadian rhythm; UMP biosynthetic process; uracil catabolic process; response to glucocorticoid stimulus; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; response to nutrient; thymidine catabolic process; pyrimidine base catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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