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DPP4 elisa kit :: Human CD26/DPP4 ELISA Kit

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Catalog # MBS176525
Unit / Price
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  96-Strip-Wells  /  $425 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CD26/DPP4, ELISA Kit

Full Product Name

Human CD26/DPP4 PicoKine ELISA Kit

Product Synonym Names
Dipeptidyl peptidase 4; dipeptidyl-peptidase 4; ADABP; ADCP-2; ADCP2; Adenosine deaminase complexing protein 2; CD26; Dipeptidyl peptidase 4; Dipeptidyl peptidase 4 soluble form; Dipeptidyl peptidase IV; Dipeptidyl peptidase IV membrane form; Dipeptidyl peptidase IV soluble form; Dipeptidylpeptidase 4; DPP 4; DPP IV; DPP4; DPP4_HUMAN; DPPIV; T-cell activation antigen CD26; TP103
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
102720
3D Structure
ModBase 3D Structure for P27487
Species Reactivity
Specificity
Natural and recombinant human CD26
Samples
Cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine
Assay Type
Sandwich
Detection Range
312pg/ml-20,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DPP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DPP4purchase
MBS176525 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD26/DPP4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPP4. The ELISA analytical biochemical technique of the MBS176525 kit is based on DPP4 antibody-DPP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DPP4 elisa kit
Principle of the assay: human CD26 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD26 has been precoated onto 96-well plates. Standards(NSO, D34-P766) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD26 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD26 amount of sample captured in plate.
Background: Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.

Typical Testing Data/Standard Curve (for reference only) of DPP4 elisa kit
Human CD26/DPP4 PicoKine ELISA Kit standard curve
DPP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS176525. Click to request current manual
NCBI/Uniprot data below describe general gene information for DPP4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
88,279 Da
NCBI Official Full Name
Dipeptidyl peptidase 4
NCBI Official Synonym Full Names
dipeptidyl-peptidase 4
NCBI Official Symbol
NCBI Official Synonym Symbols
CD26; ADABP; ADCP2; DPPIV; TP103
  [Similar Products]
NCBI Protein Information
dipeptidyl peptidase 4; ADCP-2; DPP IV; dipeptidylpeptidase 4; dipeptidyl peptidase IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
UniProt Protein Name
Dipeptidyl peptidase 4
UniProt Synonym Protein Names
ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD_antigen: CD26Cleaved into the following 2 chains:Dipeptidyl peptidase 4 membrane formAlternative name(s):Dipeptidyl peptidase IV membrane form
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ADCP2; CD26; ADCP-2; DPP IV  [Similar Products]
UniProt Entry Name
DPP4_HUMAN
NCBI Summary for DPP4
The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]
UniProt Comments for DPP4
DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily.

Protein type: Cell surface; Cell adhesion; Membrane protein, integral; Protease; EC 3.4.14.5

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: intercellular canaliculus; focal adhesion; cell surface; membrane; endocytic vesicle; lysosomal membrane; lamellipodium; apical plasma membrane; plasma membrane; integral to membrane; lipid raft

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; serine-type endopeptidase activity; receptor binding

Biological Process: regulation of cell-cell adhesion mediated by integrin; T cell activation; T cell costimulation; positive regulation of cell proliferation; response to hypoxia; endothelial cell migration; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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