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DsbA recombinant protein :: Disulfide oxidoreductase (DsbA) Recombinant Protein

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Catalog # MBS203035
Unit / Price
Scan QR to view Datasheet
  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $535 +1 FREE 8GB USB
SDS-Page
Product Name

Disulfide oxidoreductase (DsbA), Recombinant Protein

Full Product Name

Disulfide oxidoreductase (DsbA), 20-208 aa, E Coli, E Coli

Product Synonym Names
Disulfide oxidoreductase A; dsbA; rpbB; Disulfide oxidoreductase (DsbA) E Coli; DsDNA-binding protein A; Double-stranded DNA-binding protein; Disulfide oxidoreductase A periplasmic protein disulfide isomerase I; Thiol disulfide interchange protein dsbA.
Product Gene Name

DsbA recombinant protein

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sequence
MAQYEDGKQY TTLEKPVAGA PQVLEFFSFF CPHCYQFEEV LHISDNVKKK LPEGVKMTKY HVNFMGGDLG KDLTQAWAVA MALGVEDKVT VPLFEGVQKT QTIRSASDIR DVFINAGIKG EEYDAAWNSF VVKSLVAQQE KAAADVQLRG VPAMFVNGKY QLNPQGMDTS NMDVFVQQYA DTVKYLSEKK
3D Structure
ModBase 3D Structure for P0AEG4
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris pH 7.5, 2mM EDTA
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of DsbA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
DsbA recombinant protein
Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones. DsbC consists of 236 amino acids containing signal peptide (1-20 amino acids). Recombinant DsbC was expressed in E. coli and purified by using conventional chromatography techniques.
Product Categories/Family for DsbA recombinant protein
Heat Shock Proteins
Applications Tested/Suitable for DsbA recombinant protein
SDS-PAGE
SDS-Page of DsbA recombinant protein
DsbA recombinant protein SDS-Page image
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NCBI/Uniprot data below describe general gene information for DsbA. It may not necessarily be applicable to this product.
NCBI GI #
16131701
NCBI GeneID
948353
NCBI Accession #
NP_418297 [Other Products]
NCBI GenBank Nucleotide #
NC_000913.3 [Other Products]
UniProt Primary Accession #
P0AEG4 [Other Products]
UniProt Secondary Accession #
P24991; Q2M8F8; Q46951; Q46952[Other Products]
Molecular Weight
21.2kDa (190aa), confirmed by MALDI-TOF.
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NCBI Official Full Name
periplasmic protein disulfide isomerase I
NCBI Official Symbol
dsbA  [Similar Products]
NCBI Official Synonym Symbols
dsf; ECK3852; iarA; JW3832; ppfA
  [Similar Products]
NCBI Protein Information
periplasmic protein disulfide isomerase I
UniProt Protein Name
Thiol:disulfide interchange protein DsbA
Protein Family
Double-stranded DNA-binding protein
UniProt Gene Name
dsbA  [Similar Products]
UniProt Synonym Gene Names
dsf; ppfA  [Similar Products]
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NCBI Summary for DsbA
DsbA has a role in cytochrome C synthesis. [More information is available at EcoGene: EG11297].
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UniProt Comments for DsbA
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Product References and Citations for DsbA recombinant protein
Charbonnier, J. B. et al. (1999). Protein Sci. 8:96-105; Inaba, K. et al. (2004). J. Biol. Chem. 279:6761-6768.
Research Articles on DsbA
1. analysis of mutants that map to two areas in the structure of DsbB, one located between the two first transmembrane segments where the quinone ring binds and the other located in the second periplasmic loop of DsbB, which interacts with DsbA
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated withDsbA recombinant protein
 Reference Product  PubMed Publications
 dsbB recombinant protein  >147 publications with DsbA and dsbB
 phoA recombinant protein  >19 publications with DsbA and phoA
 dsbE recombinant protein  >7 publications with DsbA and dsbE
 trxB recombinant protein  >2 publications with DsbA and trxB
Pathways associated with DsbA recombinant protein
 Products by Pathway  Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway Diagram
 Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway antibodies  Cationic Antimicrobial Peptide (CAMP) Resistance, Envelope Protein Folding And Degrading Factors DegP And DsbA Pathway Diagram
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