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DSPP elisa kit :: Human dentin sialophosphoprotein ELISA Kit

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Catalog # MBS944911
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

dentin sialophosphoprotein (DSPP), ELISA Kit

Popular Item
Also Known As

Human dentin sialophosphoprotein, DSPP ELISA Kit

Product Synonym Names
Human dentin sialophosphoprotein (DSPP) ELISA kit; DFNA39; DGI1; DMP3; DPP; DSP; DTDP2; dentin phosphophoryn; dentin phosphoprotein; dentin phosphoryn; dentin sialoprotein; dentin sialophosphoprotein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS944911 Testing Data
Sequence Length
1,301
OMIM
605594
3D Structure
ModBase 3D Structure for Q9NZW4
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human DSPP. No significant cross-reactivity or interference between human DSPP and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
9.4 ng/ml -600 ng/ml.
Sensitivity
The minimum detectable dose of human DSPP is typically less than 2.35 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DSPP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDSPPpurchase
MBS944911 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the dentin sialophosphoprotein (DSPP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DSPP. The ELISA analytical biochemical technique of the MBS944911 kit is based on DSPP antibody-DSPP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DSPP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DSPP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DSPP elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DSPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DSPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSPP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSPP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of DSPP elisa kit
DSPP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS944911. Click to request current manual
NCBI/Uniprot data below describe general gene information for DSPP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
131,151 Da
NCBI Official Full Name
dentin sialophosphoprotein preproprotein
NCBI Official Synonym Full Names
dentin sialophosphoprotein
NCBI Official Symbol
NCBI Official Synonym Symbols
DPP; DSP; DGI1; DMP3; DTDP2; DFNA39
  [Similar Products]
NCBI Protein Information
dentin sialophosphoprotein; dentin phosphoryn; dentin sialoprotein; dentin phosphophoryn; dentin phosphoprotein
UniProt Protein Name
Dentin sialophosphoprotein
UniProt Synonym Protein Names
Dentin phosphophoryn; DPP
UniProt Gene Name
UniProt Synonym Gene Names
DPP; DSP  [Similar Products]
UniProt Entry Name
DSPP_HUMAN
NCBI Summary for DSPP
This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for DSPP
DSPP: DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals. Defects in DSPP are the cause of deafness autosomal dominant type 39 with dentinogenesis imperfecta 1 (DFNA39/DGI1). Affected individuals present DGI1 associated with early onset progressive sensorineural high-frequency hearing loss. Defects in DSPP are the cause of dentinogenesis imperfecta type 1 (DGI1); also known as dentinogenesis imperfecta Shields type 2 (DGI2). DGI1 is an autosomal dominant disorder in which both the primary and the permanent teeth are affected. It occurs with an incidence of 1:8000 live births. The teeth are amber and opalescent, the pulp chamber being obliterated by abnormal dentin. The enamel, although unaffected, tends to fracture, which makes dentin undergo rapid attrition, leading to shortening of the teeth. Defects in DSPP are a cause of dentinogenesis imperfecta Shields type 3 (DGI3). Patients with DGI3 do not have stigmata of osteogenesis imperfecta. The finding that a single defects in the DSPP gene causes both phenotypic patterns of DGI2 and DGI3 strongly supports the conclusion that these two disorders are not separate diseases but rather the phenotypic variation of a single genetic defect. Defects in DSPP are the cause of dentin dysplasia type 2 (DTDP2); also known as dentin dysplasia Shields type 2. DTDP2 is an autosomal dominant disorder in which mineralization of the dentine of the primary teeth is abnormal. On the basis of the phenotypic overlap between, and shared chromosomal location with DGI2 it has been proposed that DTDP2 and DGI2 are allelic. From the results of recent studies, it is clear that different types of mutations in DSPP lead to the two different phenotypes.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: collagen binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: ossification; extracellular matrix organization and biogenesis; biomineral formation; multicellular organismal development; skeletal development

Disease: Dentinogenesis Imperfecta, Shields Type Iii; Deafness, Autosomal Dominant 39, With Dentinogenesis Imperfecta 1; Dentin Dysplasia, Type Ii; Dentinogenesis Imperfecta 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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